ALDH1A1抗体

Name: ALDH1A1抗体
Brand: GeneTex
SKU: GTX123973
Price: 4000.00 RMB
Rating: 5 (2 reviews)

Anti-ALDH1A1 antibody used in IHC (Whole mount) (IHC-Wm). GTX123973

Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973

Anti-ALDH1A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX123973

Anti-ALDH1A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX123973

目录号 GTX123973

文献引用 24

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, IHC-Wm
种属反应	Human, Mouse, Rat, Zebrafish, Cat
产品包装	100 μl (¥4000), 25 μl (¥1700)

查看所有 ALDH1A1 的产品

View product citations for antibody GTX123973 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:5000-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Wm	Assay dependent

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

55 kDa. ( Note )

阳性对照

A549 , mouse liver , rat liver

预测种属反应

Rabbit, Sheep, Bovine, Dog, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human ALDH1A1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11166810

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

aldehyde dehydrogenase 1 family member A1 , ALDC , ALDH-E1 , ALDH1 , ALDH11 , HEL-9 , HEL-S-53e , HEL12 , PUMB1 , RALDH1

细胞定位

Cytoplasm

功能与背景

This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a cytosolic isoform, which has a high affinity for aldehydes. [provided by RefSeq]

数据库

Gene ID: 216 ALDH1A1
UniProt: P00352 ALDH1A1

研究领域

產品图片

GTX123973 IHC-Wm Image

ALDH1A1 antibody detects Aldh1a2 protein on whole-mount zebrafish embryos by immunohistochemical analysis.?
Sample: Paraformaldehyde-fixed zebrafish embryos.
ALDH1A1 antibody (GTX123973) dilution: 1:200.

GTX123973 WB Image

PG-4 whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A1 antibody (GTX123973) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX123973 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A1 antibody (GTX123973) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX123973 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using ALDH1A1(GTX123973) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX123973 WB Image

ALDH1A1 antibody detects ALDH1A1 protein by western blot analysis.
A. 50 μg rat liver lysate/extract
10% SDS-PAGE
ALDH1A1 antibody (GTX123973) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX123973 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using ALDH1A1(GTX123973) antibody at 1:500 dilution.

GTX123973 WB Image

ALDH1A1 antibody detects ALDH1A1 protein by western blot analysis.
A. 50 μg mouse liver lysate/extract
10% SDS-PAGE
ALDH1A1 antibody (GTX123973) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX123973 WB Image

The data was published in the journal Mol Oncol in 2020.PMID: 32037723

GTX123973 WB Image

The data was published in the 2021 in Cancer Drug Resist. PMID: 35582384

GTX123973 WB Image

The data was published in the journal Cell Death Dis in 2015. PMID: 26136074

GTX123973 WB Image

The data was published in the 2020 in Front Pharmacol. PMID: 33381038

文献引用

Application Reference

TY Chang et al. Int J Biol Sci 2024; 20 (3) : 880–896 Nε-(1-Carboxymethyl)-L-lysine/RAGE Signaling Drives Metastasis and Cancer Stemness through ERK/NFκB axis in Osteosarcoma

BY Wang et al. Am J Cancer Res 2023; 13 (10) : 4721-4733 Inhibition of Na+/H+ exchanger (NHE) 7 by 5-(N-ethyl-N-isopropyl)-Amiloride displays anti-cancer activity in non-small cell lung cancer by disrupting cancer stem cell activity and downregulating PD-L1 expression

Martinez PA et al. Brain Behav 2023; 13 (9) : e3150 Impaired aldehyde detoxification exacerbates motor deficits in an alpha-synuclein mouse model of Parkinson's disease.

Guo S et al. Cell Mol Life Sci 2023; 80 (9) : 270 TRPM7 transactivates the FOSL1 gene through STAT3 and enhances glioma stemness.

Masato A et al. NPJ Parkinsons Dis 2023; 9 (1) : 42 DOPAL initiates αSynuclein-dependent impaired proteostasis and degeneration of neuronal projections in Parkinson's disease.

实验应用 : WB, ICC/IF

种属反应 : Mouse

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产品评论

ALDH1A1 antibody 目录号 GTX123973

Rating

( Average 5 based on 2 users reviews)

实验应用

Western Blot(WB)

( Average 5 based on 2 users reviews)

Date : Anonymous submitted on 18-Jul-2016

Score :

Application Tested : WB

Sample Species : Hu

Sample : T24 cells

Amount used : 1* 10^5 cells

Blocking : 5% milk, 25°C, 1Hr

Primary Antibody : 1:1000, 4°C, 16Hr

Date : Anonymous submitted on 24-Dec-2014

Score :

Application Tested : WB

Sample Species : Hu

Sample : Huh7 cells, HepG2 cells

Amount used : 20μg

Blocking : 5% non-fat milk, 25°C, 1Hr

Primary Antibody : 1:3000, 4°C, 16Hr

条带说明 : Lane A: HepG2 cells; Lane B: Huh7 cells

我要评论产品

Application Reference

TY Chang et al. Int J Biol Sci 2024; 20 (3):880–896 Nε-(1-Carboxymethyl)-L-lysine/RAGE Signaling Drives Metastasis and Cancer Stemness through ERK/NFκB axis in Osteosarcoma

BY Wang et al. Am J Cancer Res 2023; 13 (10):4721-4733 Inhibition of Na+/H+ exchanger (NHE) 7 by 5-(N-ethyl-N-isopropyl)-Amiloride displays anti-cancer activity in non-small cell lung cancer by disrupting cancer stem cell activity and downregulating PD-L1 expression

Martinez PA et al. Brain Behav 2023; 13 (9):e3150 Impaired aldehyde detoxification exacerbates motor deficits in an alpha-synuclein mouse model of Parkinson's disease.

Guo S et al. Cell Mol Life Sci 2023; 80 (9):270 TRPM7 transactivates the FOSL1 gene through STAT3 and enhances glioma stemness.

Masato A et al. NPJ Parkinsons Dis 2023; 9 (1):42 DOPAL initiates αSynuclein-dependent impaired proteostasis and degeneration of neuronal projections in Parkinson's disease.

实验应用 : WB, ICC/IF

种属反应 : Mouse

Seung-Hun Kim et al. Cancer Drug Resist 2021; 4 (4):866-880 The aqueous extract of brucea javanica reduces tumorigenicity of human lung cancer tumorspheres.

实验应用 : WB

种属反应 : Human

Takahashi H et al. Stem Cell Res Ther 2021; 12 (1):471 Antifibrotic effect of lung-resident progenitor cells with high aldehyde dehydrogenase activity.

实验应用 : IHC-P

种属反应 : Mouse

King P et al. Int J Oncol 2021; 59 (4) Regulation of gliomagenesis and stemness through acid sensor ASIC1a.

种属反应 : Human

Morello G et al. Cancer Immunol Res 2021; 9 (7):825-837 T Cells Expressing Receptor Recombination/Revision Machinery Are Detected in the Tumor Microenvironment and Expanded in Genomically Over-unstable Models.

实验应用 : IHC-P

种属反应 : Mouse

Talaat S.M. et al. Learning Materials in Biosciences 2021; (Epub) Immunohistochemical expression of stem cell markers ALDH1 and CD44 in urothelial carcinoma of the urinary bladder.

Jingwei Wan et al. Front Pharmacol 2020; 11:590723 TRPM7 Induces Tumorigenesis and Stemness Through Notch Activation in Glioma.

实验应用 : WB

种属反应 : Human

Zhang J et al. Int J Mol Sci 2020; 21 (9) ALDH1A1 Inhibits Chicken Preadipocytes' Proliferation and Differentiation via the PPARγ Pathway In Vitro and In Vivo.

Lai HT et al. Mol Oncol 2020; 14 (6):1327-1347 GATA6 enhances the stemness of human colon cancer cells by creating a metabolic symbiosis through upregulating LRH-1 expression.

Lin YY et al. Int J Med Sci 2020; 17 (11):1639-1651 PODXL2 maintains cellular stemness and promotes breast cancer development through the Rac1/Akt pathway.

实验应用 : WB

种属反应 : Human

Kitakaze T et al. Arch Biochem Biophys 2020; 686:108329 6-(Methylsulfinyl)hexyl isothiocyanate protects acetaldehyde-caused cytotoxicity through the induction of aldehyde dehydrogenase in hepatocytes.

实验应用 : WB

种属反应 : Mouse

Fletcher PA et al. Front Endocrinol (Lausanne) 2019; 10:623 Cell Type- and Sex-Dependent Transcriptome Profiles of Rat Anterior Pituitary Cells.

实验应用 : ICC/IF

种属反应 : Rat

Castagnoli L et al. Oncogene 2019; (Epub) WNT signaling modulates PD-L1 expression in the stem cell compartment of triple-negative breast cancer.

种属反应 : Human

Chen L et al. J Cell Mol Med 2017; (Epub) TRAF6 regulates tumour metastasis through EMT and CSC phenotypes in head and neck squamous cell carcinoma.

实验应用 : WB

种属反应 : Human

Ma L et al. PLoS One 2017; 12 (11):e0188665 Suppression of cancer stemness by upregulating Ligand-of-Numb protein X1 in colorectal carcinoma.

实验应用 : WB

种属反应 : Human

Huang CF et al. J Exp Clin Cancer Res 2017; 36 (1):116 NLRP3 inflammasome activation promotes inflammation-induced carcinogenesis in head and neck squamous cell carcinoma.

实验应用 : WB

种属反应 : Mouse

Ni YL et al. Apoptosis 2017; (Epub) A potent indolylquinoline alleviates growth of human lung cancer cell tumorspheres.

实验应用 : WB

种属反应 : Human

Wu TF et al. Tumour Biol 2017; 39 (4):1.01E+15 Expression and associations of TRAF1, BMI-1, ALDH1, and Lin28B in oral squamous cell carcinoma.

实验应用 : IHC-P

种属反应 : Human

Yan L et al. Sci Rep 2016; 6:28281 MiR-21-5p Links Epithelial-Mesenchymal Transition Phenotype with Stem-Like Cell Signatures via AKT Signaling in Keloid Keratinocytes.

实验应用 : WB

种属反应 : Human

Ju SY et al. Cell Death Dis 2015; 6 (Epub):e1801 identification of * as a novel therapeutic agent that targets human colon cancer stem cells.

实验应用 : WB

种属反应 : Human

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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ALDH1A1抗体

目录号 GTX123973

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX123973 IHC-Wm Image

GTX123973 WB Image

GTX123973 WB Image

GTX123973 IHC-P Image

GTX123973 WB Image

GTX123973 ICC/IF Image

GTX123973 WB Image

GTX123973 WB Image

GTX123973 WB Image

GTX123973 WB Image

GTX123973 WB Image

文献引用

Application Reference

产品评论

ALDH1A1 antibody 目录号 GTX123973

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