产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:-1:10000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
human AXL-transfected 293T , HeLa
预测种属反应
Mouse, Rat, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.6 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human AXL. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2885984
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
AXL receptor tyrosine kinase , ARK , JTK11 , Tyro7 , UFO
细胞定位
Membrane; Single-pass type I membrane protein
功能与背景
The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, this protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX129407 IHC-P Image
AXL antibody detects AXL protein at cell membrane in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Green: AXL antibody (GTX129407) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX129407 IHC-P Image
AXL antibody detects AXL protein at membrane on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
AXL antibody (GTX129407) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX129407 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (60 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AXL antibody (GTX129407) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX129407 IHC-P Image
AXL antibody detects AXL protein at membrane on human non-small cell lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human non-small cell lung carcinoma.
AXL antibody (GTX129407) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX129407 WB Image
AXL antibody detects AXL protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of human AXL-transfected 293T cells
7.5 % SDS-PAGE
AXL antibody (GTX129407) dilution: 1:5000
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GTX129407 IHC-P Image
AXL antibody detects AXL protein at cell membrane on human non-small cell lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human non-small cell lung carcinoma.
AXL antibody (GTX129407) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX129407 ELISA Image
An ELISA plate is coated with 50 μL of AXL recombinant protein at concentration of 4 μg/mL. The coated protein is detected with anti-AXL antibody (GTX129407) at concentration ranged from 0.25 to 1000 ng/mL.
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GTX129407 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with AXL antibody (GTX129407) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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