GeneTex中国官方网站
  • China

Aurora A抗体 [C3], C-term

Overexpression
Anti-Aurora A antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104620
Anti-Aurora A antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104620
Anti-Aurora A antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104620
Anti-Aurora A antibody [C3], C-term used in Western Blot (WB). GTX104620
Anti-Aurora A antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104620
Anti-Aurora A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104620

目录号 GTX104620

文献引用 2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Aurora A 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

46 kDa. ( Note )

预测种属反应

Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Aurora A. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1949708

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

aurora kinase A , AIK , ARK1 , AURA , BTAK , PPP1R47 , STK15 , STK6 , STK7

细胞定位

Centrosome , Spindle

功能与背景

The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Aurora A antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104620

GTX104620 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using Aurora A(GTX104620) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

Anti-Aurora A antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104620

GTX104620 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed 293T, using Aurora A(GTX104620) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Anti-Aurora A antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104620

GTX104620 ICC/IF Image

Aurora A antibody [C3], C-term detects Aurora A protein at spindle by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Aurora A stained by Aurora A antibody [C3], C-term (GTX104620) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Aurora A antibody [C3], C-term used in Western Blot (WB). GTX104620

GTX104620 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aurora A antibody [C3], C-term (GTX104620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Aurora A antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104620

GTX104620 ICC/IF Image

Aurora A antibody [C3], C-term detects Aurora A protein at spindle by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Aurora A stained by Aurora A antibody [C3], C-term (GTX104620) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Aurora A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104620

GTX104620 IHC-P Image

Immunohistochemical analysis of paraffin-embedded TW2.6 xenograft, using Aurora A(GTX104620) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

文献引用

Application Reference

Zhang Y et al. Pancreatology 2022; 22 (5) : 619-625 Aurora kinase a inhibitor MLN8237 suppresses pancreatic cancer growth.
Liu YH et al. Phytomedicine 2018; 46 : 184-192 Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.
实验应用 : WB
种属反应 : Human
我要回报文献

产品评论

目前没有使用 Aurora A antibody [C3], C-term (GTX104620) 的产品评论被收录。快发表第一篇产品评论吧！
我要评论产品
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com