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Bad抗体

KO/KD Orthogonal Overexpression
Anti-Bad antibody used in Western Blot (WB). GTX130108
Anti-Bad antibody used in Western Blot (WB). GTX130108
Anti-Bad antibody used in Western Blot (WB). GTX130108
Anti-Bad antibody used in Western Blot (WB). GTX130108
Anti-Bad antibody used in IHC (Paraffin sections) (IHC-P). GTX130108

目录号 GTX130108

文献引用 8
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, IHC-P

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

18 kDa. ( Note )

阳性对照

293T , A431 , HeLa , BAD-transfected 293T

预测种属反应

Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.36 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Bad. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2886180

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

BCL2 associated agonist of cell death , BBC2 , BCL2L8

细胞定位

Mitochondrion outer membrane , Cytoplasm

功能与背景

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Bad antibody used in Western Blot (WB). GTX130108

GTX130108 WB Image

KO/KD

Wild-type (WT) and Bad knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bad antibody (GTX130108) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Bad antibody used in Western Blot (WB). GTX130108

GTX130108 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Bad antibody (GTX130108) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bad antibody used in Western Blot (WB). GTX130108

GTX130108 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bad antibody (GTX130108) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bad antibody used in Western Blot (WB). GTX130108

GTX130108 WB Image

Bad antibody detects Bad protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bad antibody (GTX130108) diluted at a dilution of 1:1000.

Anti-Bad antibody used in IHC (Paraffin sections) (IHC-P). GTX130108

GTX130108 IHC-P Image

Bad antibody detects Bad protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Bad stained by Bad antibody (GTX130108) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

Application Reference

Chua HH et al. Cell Mol Gastroenterol Hepatol 2023; 15 (1) : 121-152 PIM1-Induced Cytoplasmic Expression of RBMY Mediates Hepatocellular Carcinoma Metastasis.
实验应用 : WB
种属反应 : Human
Lu CC et al. Int J Oncol 2019; 54 (4) : 1271-1281
Wei GJ et al. AAPS J 2019; 21 (5) : 86 A Tangeretin Derivative Inhibits the Growth of Human Prostate Cancer LNCaP Cells by Epigenetically Restoring p21 Gene Expression and Inhibiting Cancer Stem-like Cell Proliferation.
实验应用 : WB
种属反应 : Human
Lee CF et al. Biomedicine (Taipei) 2018; 8 (3) : 15 benzyl isothiocyanate (bitc) triggers mitochondria-mediated apoptotic machinery in human *-resistant oral cancer car cells.
实验应用 : WB
种属反应 : Human
Lee CC et al. PLoS One 2017; 12 (3) : e0173890 the microrna-302b-inhibited *-like growth factor-binding protein 2 signaling pathway induces glioma cell apoptosis by targeting nuclear factor ia.
实验应用 : WB
种属反应 : Human
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产品评论

Bad antibody 目录号 GTX130108

Rating
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实验应用
Western Blot(WB)
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Anti-Bad antibody used in Western Blot (WB). GTX130108
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
条带说明 : untreated-30 μM cisplatin treatment for 24hr.
我要评论产品
Application Reference
Chua HH et al. Cell Mol Gastroenterol Hepatol 2023; 15 (1):121-152 PIM1-Induced Cytoplasmic Expression of RBMY Mediates Hepatocellular Carcinoma Metastasis.
实验应用 : WB
种属反应 : Human
Lu CC et al. Int J Oncol 2019; 54 (4):1271-1281
Wei GJ et al. AAPS J 2019; 21 (5):86 A Tangeretin Derivative Inhibits the Growth of Human Prostate Cancer LNCaP Cells by Epigenetically Restoring p21 Gene Expression and Inhibiting Cancer Stem-like Cell Proliferation.
实验应用 : WB
种属反应 : Human
Lee CF et al. Biomedicine (Taipei) 2018; 8 (3):15 benzyl isothiocyanate (bitc) triggers mitochondria-mediated apoptotic machinery in human *-resistant oral cancer car cells.
实验应用 : WB
种属反应 : Human
Eid W et al. J Cell Biochem 2017; (Epub) neferine enhances the antitumor effect of *-c in hela cells through the activation of p38-mapk pathway.
实验应用 : WB
种属反应 : Human
Lee CC et al. PLoS One 2017; 12 (3):e0173890 the microrna-302b-inhibited *-like growth factor-binding protein 2 signaling pathway induces glioma cell apoptosis by targeting nuclear factor ia.
实验应用 : WB
种属反应 : Human
Chen MF et al. BMC Cancer 2016; 16:532 Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent and mitochondrial pathways in mammalian hepatic stellate cells.
实验应用 : WB
种属反应 : Human
Chen PH et al. Neuropharmacology 2016; 110 (Pt A):362-75 The miR-204-3p-targeted IGFBP2 pathway is involved in xanthohumol-induced glioma cell apoptotic death.
实验应用 : WB
种属反应 : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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