产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
mouse testis
预测种属反应
Human, Rat, Bovine, Dog(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.26 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Brachyury. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2887073
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
T-box transcription factor T , SAVA , T , TFT
细胞定位
Nucleus
功能与背景
The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX133714 IHC-P Image
Brachyury antibody detects Brachyury protein at nucleus in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Brachyury antibody (GTX133714) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX133714 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membranes were blotted with Brachyury antibody (GTX133714) diluted at 1:1000 and competitor's antibody (sc-17743) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX133714 IHC-P Image
Brachyury antibody detects Brachyury protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 mouse embryo.Green: Brachyury stained by Brachyury antibody (GTX133714) diluted at 1:250.Red: SOX2, a nucleus marker, stained by SOX2 antibody [GT1352] (GTX627405) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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