产品说明
摘要
The SK1 antibody is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.The SK1 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells. It is cross-reactive with CD8 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus. If used together with an alternative Anti-Human CD8a clone, RPA-T8, the SK1 antibody will not block binding of RPA-T8 to CD8a.
产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
0.125 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
产品属性
形式
Liquid
存储溶液
10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
浓度
0.025 mg/ml (Please refer to the vial label for the specific concentration.)
纯化方式
Purified by affinity chromatography
From tissue culture supernatant
偶联
Fluorescein isothiocyanate (FITC)
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
CD8 , CD8A , CD8a molecule , Leu2 , MAL , p32 , CD8 alpha , CD8B , CD8B1 , CD8b molecule , LEU2 , LY3 , LYT3 , P37 , CD8 beta
细胞定位
Cell membrane,Secreted
数据库
研究领域
產品图片
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GTX01468-06 FACS Image
FACS analysis of human peripheral blood lymphocytes using GTX01468-06 CD8 antibody [SK1] (FITC). Solid lone : primary antibody Dashed line : isotype control antibody amount : 0.125 μg (5 μl)
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文献引用
产品评论
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