产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Rat, Cat, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.15 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the Extracellular domain of human CD9. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2909839
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
CD9 molecule , BTCC-1 , DRAP-27 , MIC3 , MRP-1 , TSPAN-29 , TSPAN29
细胞定位
Cell membrane,Membrane
功能与背景
This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]
数据库
研究领域
產品图片
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GTX135296 ICC/IF Image
CD9 antibody detects CD9 protein at cell membrane by immunofluorescent analysis.Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CD9 stained by CD9 antibody (GTX135296) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX135296 ICC/IF Image
CD9 antibody detects CD9 protein at cell membrane by immunofluorescent analysis.Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CD9 stained by CD9 antibody (GTX135296) diluted at 1:500.
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GTX135296 IHC-P Image
CD9 antibody detects CD9 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.CD9 stained by CD9 antibody (GTX135296) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX135296 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD9 antibody (GTX135296) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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