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Cas9抗体 [7A9]

Anti-Cas9 antibody [7A9] used in Immunoprecipitation (IP). GTX53807
Anti-Cas9 antibody [7A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53807

目录号 GTX53807

文献引用 4

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 7A9

同种型

 IgG1

实验应用

 WB, ICC/IF, IP
产品包装
25 μg (¥2800)
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产品应用

应用说明

Western Blotting Analysis: 0.5 μg/mL from a representative lot detected Cas9 in 1 μg of Myc-Cas9 expressing HEK293 cell lysate. Immunoprecipitation Analysis: A representative lot immunoprecipitated Cas9 in Flag-Cas9 and Myc-Cas9 expressing cell lysate (performed by an independent laboratory).

产品说明

This antibody recognises both Cas9 and dCas9 (nuclease deficient Cas9)

产品属性

形式

Liquid

存储溶液

100mM Tris-Glycine, 150mM NaCl

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

浓度

Batch dependent (Please refer to the vial label for the specific concentration.)

免疫原

N-Terminal domain of Cas9.

纯化方式

Protein G purified

偶联

Unconjugated

RRID

AB_2887823

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

研究领域

產品图片

Anti-Cas9 antibody [7A9] used in Immunoprecipitation (IP). GTX53807

GTX53807 IP Image

IP analysis was performed with lysate from HEK293T cells expressing Flag-tagged S. pyogenes Cas9 and either Cas9 antibody [7A9] followed by incubation with a 1:1 mixture of protein A/G sepharose beads (Cas9 Sup) or Cas9 antibody [7A9] crosslinked with a 1:1 mixture of protein A/G sepharose beads (Cas9 X-link). The precipitates were detected by the same antibody. Asterisk indicates IgG heavy chain.

Anti-Cas9 antibody [7A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53807

GTX53807 ICC/IF Image

ICC/IF analysis of HeLa cells transiently transfected with flag-tagged Cas9 using Cas9 antibody [7A9] at a dilution of 0.5 μg/ml (green) and DAPI (blue).

文献引用

Application Reference

Zhao C et al. Nat Commun 2020; 11 (1) : 5178 CRISPR screening of porcine sgRNA library identifies host factors associated with Japanese encephalitis virus replication.
Shen CC et al. Nucleic Acids Res 2018; (Epub) Synthetic switch to minimize CRISPR off-target effects by self-restricting Cas9 transcription and translation.
实验应用 : WB
Germano G et al. Nature 2017; 552 (7683) : 116-120 Inactivation of DNA repair triggers neoantigen generation and impairs tumour growth.
实验应用 : WB
Guo J et al. Protein Cell 2017; (Epub) An inducible CRISPR-ON system for controllable gene activation in human pluripotent stem cells.
实验应用 : WB
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产品包装 报价 (¥)
¥ 2800
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