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Caspase 1抗体 [N1N3]

Orthogonal
Anti-Caspase 1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101322
Anti-Caspase 1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101322
Anti-Caspase 1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101322
Anti-Caspase 1 antibody [N1N3] used in Western Blot (WB). GTX101322
Anti-Caspase 1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101322
Anti-Caspase 1 antibody [N1N3] used in Western Blot (WB). GTX101322

目录号 GTX101322

文献引用 6
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

45 kDa. ( Note )

阳性对照

THP-1

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.08 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Caspase 1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10618781

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

caspase 1 , ICE , IL1BC , P45

细胞定位

Cytoplasm

功能与背景

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Caspase 1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101322

GTX101322 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Caspase 1 alpha(GTX101322) antibody at 1:500 dilution.

Anti-Caspase 1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101322

GTX101322 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Caspase 1 alpha(GTX101322) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Caspase 1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101322

GTX101322 IHC-P Image

Caspase 1 antibody [N1N3] detects Caspase 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Caspase 1 stained by Caspase 1 antibody [N1N3] (GTX101322) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Caspase 1 antibody [N1N3] used in Western Blot (WB). GTX101322

GTX101322 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Caspase 1 antibody [N1N3] (GTX101322) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Caspase 1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101322

GTX101322 IHC-P Image

Orthogonal

The data was published in the journal Evid Based Complement Alternat Med in 2013. PMID: 23653660

Anti-Caspase 1 antibody [N1N3] used in Western Blot (WB). GTX101322

GTX101322 WB Image

Orthogonal

The data was published in the journal Evid Based Complement Alternat Med in 2013. PMID: 23653660

文献引用

Application Reference

Hosoya T et al. J Am Soc Nephrol 2022; 33 (2) : 326-341 Xanthine Oxidoreductase Inhibitors Suppress the Onset of Exercise-Induced AKI in High HPRT Activity Urat1-Uox Double Knockout Mice.
实验应用 : WB
种属反应 : Mouse
Feng M et al. Int J Mol Sci 2022; 23 (17) Anti-Inflammation and Anti-Pyroptosis Activities of Mangiferin via Suppressing NF-κB/NLRP3/GSDMD Signaling Cascades.
实验应用 : WB
种属反应 : Mouse
Zhang C et al. Mol Med Rep 2021; 23 (5) Cytokine secretion and pyroptosis of cholesteatoma keratinocytes mediated by AIM2 inflammasomes in response to cytoplasmic DNA.
实验应用 : WB, IHC-P
种属反应 : Human
Wu CY et al. J Cell Mol Med 2020; (Epub) Tris DBA ameliorates IgA nephropathy by blunting the activating signal of NLRP3 inflammasome through SIRT1- and SIRT3-mediated autophagy induction.
实验应用 : WB
种属反应 : Mouse
Chen C.Y. et al. Clinical Science 2018; 132 (20) : 2221-2239 Omega-3 Polyunsaturated Fatty Acids Reduce Preterm Labor by Inhibiting Trophoblast Cathepsin S and Inflammasome Activation.
实验应用 : WB
种属反应 : Human
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Caspase 1 antibody [N1N3] 目录号 GTX101322

Rating
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实验应用
Western Blot(WB)
( Average 3 based on 1 users reviews)
Anti-Caspase 1 antibody [N1N3] used in Western Blot (WB). GTX101322
Date : Anonymous submitted on 14-Feb-2023
Score :
Application Tested : WB
Sample Species : Ms
Sample : Mouse brain tissue lysate
Amount used : 10μg
Blocking : 5% non-fat milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:200, 16°C, 25Hr
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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