产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human DHCR7.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
7-dehydrocholesterol reductase , SLOS
细胞定位
Endoplasmic reticulum membrane
功能与背景
This gene encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. This gene is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by cognitive disability, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Aug 2009]
数据库
研究领域
產品图片
![Anti-DHCR7 antibody [HL2764] used in Western Blot (WB). GTX639629](/templates/default/js/lightbox/images/loading.gif "Anti-DHCR7 antibody [HL2764] used in Western Blot (WB). GTX639629")
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GTX639629 WB Image
MCF-7 cytosol and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DHCR7 antibody [HL2764] (GTX639629) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (Cyto: cytosol extract; ME: membrane extract)
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GTX639629 WB Image
Non-transfected (–) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DHCR7 antibody [HL2764] (GTX639629) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-DHCR7 antibody [HL2764] used in Western Blot (WB). GTX639629](/upload/website/prouct_img/small/GTX639629/GTX639629_45404_20240510_WB_Fraction_24052202_524.webp "Anti-DHCR7 antibody [HL2764] used in Western Blot (WB). GTX639629")
![Anti-DHCR7 antibody [HL2764] used in Western Blot (WB). GTX639629](/upload/website/prouct_img/small/GTX639629/GTX639629_T-45292_20240315_WB_B_24031823_335.webp "Anti-DHCR7 antibody [HL2764] used in Western Blot (WB). GTX639629")
![Anti-DHCR7 antibody [HL2764] used in Western Blot (WB). GTX639629](/upload/website/prouct_img/normal/GTX639629/GTX639629_45404_20240510_WB_Fraction_24052202_524.webp "Anti-DHCR7 antibody [HL2764] used in Western Blot (WB). GTX639629")
![Anti-DHCR7 antibody [HL2764] used in Western Blot (WB). GTX639629](/upload/website/prouct_img/normal/GTX639629/GTX639629_T-45292_20240315_WB_B_24031823_335.webp "Anti-DHCR7 antibody [HL2764] used in Western Blot (WB). GTX639629")
