产品说明
摘要
E-Cadherin antibody detects E-cadherin protein, which is encoded by the CDH1 gene. E-cadherin is a calcium-dependent cell adhesion protein (predicted molecular weight of 97 kDa) that plays a vital role in cell migration and proliferation. Because E-cadherin is an epithelial cell marker, this antibody is a well-published and widely used reagent to study E-cadherin protein expression during the epithelial-mesenchymal transition (EMT) in various physiological and pathological processes.
产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
| 1:100-1:500 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
MCF-7
预测种属反应
Bovine, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.14 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_10729586
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO
细胞定位
Cell junction , Cell membrane
功能与背景
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX100443 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with E-Cadherin antibody (GTX100443) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX100443 WB Image
Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100443 IHC-P Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat duodenum.E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX100443 IP Image
E-cadherin antibody immunoprecipitates E-cadherin protein in IP experiments.
IP samples: MCF-7 whole cell extract
A. Control with 3 μg of preimmune Rabbit IgG
B. Immunoprecipitation of E-cadherin protein by 3 μg E-cadherin antibody (GTX100443)
5 % SDS-PAGE
The immunoprecipitated E-cadherin protein was detected by E-cadherin antibody (GTX100443) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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GTX100443 ICC/IF Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10 μm.
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GTX100443 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human ulcerative colitis tissue using E-Cadherin antibody (GTX100443).
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GTX100443 IHC-P Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
E-Cadherin antibody (GTX100443) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX100443 IHC-P Image
E-cadherin antibody detects E-cadherin protein at membrane on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
E-cadherin antibody (GTX100443) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100443 IHC-P Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
E-Cadherin antibody (GTX100443) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX100443 IHC-P Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
E-Cadherin antibody (GTX100443) diluted at 1:400.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX100443 ICC/IF Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin protein stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX100443 IHC-P Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
E-Cadherin antibody (GTX100443) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX100443 IHC-P Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
E-Cadherin antibody (GTX100443) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX100443 IHC-P Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX100443 ICC/IF Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin protein stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX100443 IHC-P Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX100443 IHC-P Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded dog skin.E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX100443 ICC/IF Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
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GTX100443 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100443 WB Image
Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100443 ICC/IF Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
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GTX100443 IP Image
Immunoprecipitation of E-Cadherin protein from MCF-7 whole cell extracts using 5 μg of E-Cadherin antibody (GTX100443).
Western blot analysis was performed using E-Cadherin antibody (GTX100443).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX100443 WB Image
Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100443 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100443 WB Image
The data was published in the 2022 in Front Pharmacol. PMID: 35517780
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GTX100443 WB Image
The data was published in the journal Front Oncol in 2020.PMID: 32211324
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GTX100443 WB Image
The data was published in the 2022 in Front Pharmacol. PMID: 35517780
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GTX100443 WB Image
The data was published in the 2022 in Front Pharmacol. PMID: 35517780
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GTX100443 IHC-P Image
The data was published in the journal Cell Death Dis in 2020.PMID: 32080178
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GTX100443 WB Image
The data was published in the journal Int J Mol Sci in 2019.PMID: 31426484
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GTX100443 IHC-P Image
The data was published in the journal Evid Based Complement Alternat Med in 2019. PMID: 31186661
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GTX100443 WB Image
The data was published in the journal Evid Based Complement Alternat Med in 2019. PMID: 31186661
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GTX100443 WB Image
The data was published in the journal PLoS One in 2017. PMID: 28683123
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GTX100443 WB Image
The data was published in the journal PLoS One in 2017. PMID: 28683123
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GTX100443 WB Image
The data was published in the journal Sci Rep in 2017. PMID: 28195146
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GTX100443 WB Image
The data was published in the journal Front Endocrinol (Lausanne) in 2017. PMID: 29109696
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GTX100443 WB Image
The data was published in the 2022 in Front Pharmacol. PMID: 35517780
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文献引用
产品评论
E-Cadherin antibody
目录号 GTX100443
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Rating
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( Average 4.8 based on 9 users reviews)
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实验应用
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Western Blot(WB)
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( Average 4.7 based on 6 users reviews)
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Immunocytochemistry/ Immunofluorescence(ICC/IF)
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( Average 5 based on 1 users reviews)
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IHC (Paraffin sections)(IHC-P)
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( Average 5 based on 2 users reviews)
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Date :
Anonymous submitted on 02-May-2024
Application Tested :
IHC-P
Sample Species :
Ms
Sample :
Mouse liver tissue
Fixation :
4% PFA in PBS
Permeabilization /Antigen retrieval :
Citrate buffer, pH 6.0, 0.25Hr
Primary Antibody :
1:1000, 4°C, 16-18Hr
Date :
Anonymous submitted on 10-Oct-2023
Application Tested :
IHC-P
Sample Species :
Ms
Sample :
Mouse Brain tissue
Blocking :
0.5% skim milk in PBST
Fixation :
4% PFA
Permeabilization /Antigen retrieval :
PBS-T / Microwave heating in citrate buffer
Primary Antibody :
1:1000, 4°C, 18Hr
Date :
Anonymous submitted on 27-Jan-2022
Application Tested :
WB
Sample Species :
Hu
Sample :
MDA-MB-231 cells, MCF-7 cells
Amount used :
30μg
Blocking :
5% non-fat milk in PBS, 4°C, 1.5Hr
Primary Antibody :
1:3000, 4°C, > 12Hr
Date :
Anonymous submitted on 22-Sep-2021
Application Tested :
ICC/IF
Sample Species :
Hu
Sample :
A549 cells
Fixation :
4% PFA
Permeabilization /Antigen retrieval :
0.2% Triton X-100 in PBS, RT, 0.5Hr
Primary Antibody :
1:100, 4°C, 16Hr
Date :
Anonymous submitted on 22-Feb-2021
Application Tested :
WB
Sample Species :
Ms
Sample :
colonic mucosa
Amount used :
25 μg
Blocking :
5% fat dry milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Date :
Anonymous submitted on 30-Dec-2020
Application Tested :
WB
Sample Species :
Hu
Sample :
A549 cells
Amount used :
80 μg
Blocking :
1% skim milk, 24°C, 1Hr
Primary Antibody :
1:2000, 24°C, 2Hr
Date :
Anonymous submitted on 21-Jun-2020
Application Tested :
WB
Sample Species :
Hu
Sample :
oecm1 cells
Amount used :
20 μg
Blocking :
10% non-fat milk, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 24Hr
Date :
Anonymous submitted on 26-May-2018
Application Tested :
WB
Sample Species :
Hu
Amount used :
30 μg
Blocking :
5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
条带说明 :
Lane 1: hFOB1.19 cells, Lane 2: MG63 cells, Lane 3: U2OS cells
Date :
Anonymous submitted on 24-Apr-2015
Application Tested :
WB
Sample Species :
Hu
Sample :
HT-29 cells
Amount used :
30μg
Blocking :
5% skim milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
