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EGFR抗体 [GT133]

KO/KD Comparison Orthogonal Overexpression IP/MS
Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887
Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887
Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887
Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887
Anti-EGFR antibody [GT133] used in IHC (Paraffin sections) (IHC-P). GTX628887
Anti-EGFR antibody [GT133] used in IHC (Paraffin sections) (IHC-P). GTX628887
Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887
Anti-EGFR antibody [GT133] used in Immunoprecipitation (IP). GTX628887
Anti-EGFR antibody [GT133] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628887
Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887

目录号 GTX628887

文献引用 4

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT133

同种型

 IgG2b

实验应用

 WB, ICC/IF, IHC-P, IP

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品说明

摘要

EGFR antibody recognizes epidermal growth factor receptor (EGFR, also known as HER1 in humans), a receptor tyrosine kinase with a predicted molecular weight of 134 kDa. EGFR is involved in epithelial tissue development and homeostasis. It is also a driver of tumorigenesis. Upregulation or abnormal activation of EGFR is frequently found in glioblastomas, lung cancers, and breast cancers, among others.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

134 kDa. ( Note )

Observed MW

180 kDa.

阳性对照

A431 , A549 , H1299 , HCT116

产品说明

This antibody was raised against human EGFR Intracellular domain.This antibody does not cross-react with Her2/Erbb2 protein.
IP/MS validation was supported by references (PMID:30377423)

预测种属反应

Mouse, Rat, Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the Intracellular domain of human EGFR. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888064

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA

细胞定位

Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted

功能与背景

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887

GTX628887 WB Image

KO/KD

Non-transfected (–) and transfected (+) A431 whole cell extracts (15 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT133] (GTX628887) diluted at 1:8000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887

GTX628887 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with EGFR antibody [GT133] (GTX628887) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887

GTX628887 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT133] (GTX628887) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887

GTX628887 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT133] (GTX628887) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-EGFR antibody [GT133] used in IHC (Paraffin sections) (IHC-P). GTX628887

GTX628887 IHC-P Image

EGFR antibody [GT133] detects EGFR protein at cell membrane on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
EGFR antibody [GT133] (GTX628887) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EGFR antibody [GT133] used in IHC (Paraffin sections) (IHC-P). GTX628887

GTX628887 IHC-P Image

EGFR antibody [GT133] detects EGFR protein at cell membrane on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
EGFR antibody [GT133] (GTX628887) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887

GTX628887 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT133] (GTX628887) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-EGFR antibody [GT133] used in Immunoprecipitation (IP). GTX628887

GTX628887 IP Image

EGFR antibody [GT133] immunoprecipitates EGFR protein in IP experiments.
IP samples: A431 whole cell extract
A. 40 μg A431 whole cell extract
B. Control with 4 μg of preimmune Mouse IgG
C. Immunoprecipitation of EGFR protein by 4 μg EGFR antibody [GT133] (GTX628887)
5 % SDS-PAGE
The immunoprecipitated EGFR protein was detected by EGFR antibody [GT133] (GTX628887) diluted at 1:5000.
[EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent]
Anti-EGFR antibody [GT133] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628887

GTX628887 ICC/IF Image

EGFR antibody [GT133] detects EGFR protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EGFR stained by EGFR antibody [GT133] (GTX628887) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887

GTX628887 WB Image

Orthogonal

The data was published in the 2022 in J Oncol. PMID: 35646111

文献引用

Application Reference

Pang HH et al. ACS Nano 2023; 17 (11) : 10407-10422 Bioengineered Bacteriophage-Like Nanoparticles as RNAi Therapeutics to Enhance Radiotherapy against Glioblastomas.
Ching-Ann Liu et al. J Oncol 2022; 2022 : 3236058 Targeting the Axl and mTOR Pathway Synergizes Immunotherapy and Chemotherapy to Butylidenephthalide in a Recurrent GBM.
Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
实验应用 : IP
种属反应 : Human
Baykul T et al. J Res Med Den Sci 2016; 4 (1) : 25-29 EGFR Expression and Incidence of Cystic Changes in Asymptomatic Dental Follicles in Smoking Patients.
实验应用 : IHC-P
种属反应 : Human
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