产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:1000-1:10000 |
1:100-1:1000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Bovine, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human GFAP. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2910057
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
glial fibrillary acidic protein , ALXDRD
细胞定位
Cytoplasm
功能与背景
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
数据库
研究领域
產品图片
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GTX636725 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with GFAP antibody [HL1307] (GTX636725) diluted at 1:3000 and competitor's antibody (CST 34001) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX636725 IHC-P Image
GFAP antibody [HL1307] detects GFAP protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissue.GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636725 IHC-P Image
GFAP antibody [HL1307] detects GFAP protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636725 IHC-Fr Image
GFAP antibody [HL1307] detects GFAP protein at glia cells by immunohistochemical analysis.Sample: Frozen-sectioned mouse cerebellum.Green: GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:200.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636725 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody [HL1307] (GTX636725) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636725 IHC-P Image
GFAP antibody [HL1307] detects GFAP protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636725 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody [HL1307] (GTX636725) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636725 WB Image
Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody [HL1307] (GTX636725) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636725 IHC-Fr Image
GFAP antibody [HL1307] detects GFAP protein at cytoplasm by immunohistochemical analysis.Sample: Frozen-sectioned mouse spinal cord.Green: GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636725 IHC-P Image
GFAP antibody [HL1307] detects GFAP protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636725 IHC-P Image
GFAP antibody [HL1307] detects GFAP protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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