Glutamine synthetase抗体 [GT1055]

Name: Glutamine synthetase抗体 [GT1055]
Brand: GeneTex
SKU: GTX630654
Price: 4000.00 RMB

Anti-Glutamine synthetase antibody [GT1055] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630654

Anti-Glutamine synthetase antibody [GT1055] used in IHC (Frozen sections) (IHC-Fr). GTX630654

Anti-Glutamine synthetase antibody [GT1055] used in Western Blot (WB). GTX630654

Anti-Glutamine synthetase antibody [GT1055] used in IHC (Paraffin sections) (IHC-P). GTX630654

目录号 GTX630654

文献引用 3

宿主	Mouse
克隆	Monoclonal
克隆编号	GT1055
同种型	IgG1
实验应用	WB, ICC/IF, IHC-P, IHC-Fr
种属反应	Human, Mouse, Rat
产品包装	100 μl (¥4000), 25 μl (¥1700)

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View product citations for antibody GTX630654 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

42 kDa. ( Note )

阳性对照

U87-MG , SK-N-SH , IMR32 , SK-N-AS , 293T , HeLa , HepG2 , mouse eye , mouse brain , rat brain , DDDDK-tagged GLUL-transfected 293T

预测种属反应

Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Full length human Glutamine synthetase Recombinant protein.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888230

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

glutamate-ammonia ligase , GLNS , GS , PIG43 , PIG59

细胞定位

Cytoplasm

功能与背景

Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling (Haberle et al., 2005 [PubMed 16267323]). Fetal glutamine requirements are very high and depend largely on active glutamine synthesis and the release of glutamine into the fetal circulation by the placenta. Glutamine synthetase (EC 6.3.1.2), also called glutamate-ammonia ligase (GLUL), is expressed throughout the body and plays an important role in controlling body pH and in removing ammonia from the circulation. The enzyme clears L-glutamate, the major neurotransmitter in the central nervous system, from neuronal synapses (see references in Clancy et al., 1996 [PubMed 8975719]).[supplied by OMIM]

数据库

Gene ID: 2752 GLUL
UniProt: P15104 GLUL

研究领域

產品图片

GTX630654 ICC/IF Image

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytoplasm by immunofluorescent analysis.
Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:250.
Red: GFAP, stained by GFAP antibody (GTX27260) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX630654 IHC-Fr Image

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:250.
Red: PKC alpha protein stained by PKC alpha antibody [GT1876] (GTX130453) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX630654 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:5000.

GTX630654 WB Image

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
Glutamine synthetase antibody [GT1055] (GTX630654) dilution: 1:1000

GTX630654 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX630654 IHC-Fr Image

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:500. Blue: DAPI

GTX630654 WB Image

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein by western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
B. 30 μg SK-N-SH whole cell lysate/extract
C. 30 μg IMR32 whole cell lysate/extract
D. 30 μg SK-N-AS whole cell lysate/extract
10 % SDS-PAGE
Glutamine synthetase antibody [GT1055] (GTX630654) dilution: 1:1000

GTX630654 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX630654) diluted at 1:1000.

GTX630654 IHC-P Image

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Glutamine synthetase antibody [GT1055] (GTX630654) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX630654 IHC-P Image

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatoma.
Glutamine synthetase antibody [GT1055] (GTX630654) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX630654 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:5000.

GTX630654 IHC-P Image

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytosol on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
Glutamine synthetase antibody [GT1055] (GTX630654) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

文献引用

Application Reference

Jin-Wei Jhu et al. Int J Mol Sci 2021; 22 (18) SREBP1-Induced Glutamine Synthetase Triggers a Feedforward Loop to Upregulate SREBP1 through Sp1 O-GlcNAcylation and Augments Lipid Droplet Formation in Cancer Cells.

实验应用 : WB

种属反应 : Human

Lin D et al. Cells 2021; 10 (4)

实验应用 : WB

种属反应 : Human

Seki T et al. Mol Cell Neurosci 2018; 93 : 36-47 d-Cysteine promotes dendritic development in primary cultured cerebellar Purkinje cells via hydrogen sulfide production.

实验应用 : ICC/IF

种属反应 : Rat

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Application Reference

实验应用 : WB

种属反应 : Human

Lin D et al. Cells 2021; 10 (4)

实验应用 : WB

种属反应 : Human

Seki T et al. Mol Cell Neurosci 2018; 93:36-47 d-Cysteine promotes dendritic development in primary cultured cerebellar Purkinje cells via hydrogen sulfide production.

实验应用 : ICC/IF

种属反应 : Rat

SDS
PBS.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
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	http://www.neobioscience.com
	genetex@neobioscience.com

Glutamine synthetase抗体 [GT1055]

目录号 GTX630654

宿主

克隆

克隆编号

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX630654 ICC/IF Image

GTX630654 IHC-Fr Image

GTX630654 WB Image

GTX630654 WB Image

GTX630654 WB Image

GTX630654 WB Image

GTX630654 IHC-Fr Image

GTX630654 WB Image

GTX630654 WB Image

GTX630654 IHC-P Image

GTX630654 IHC-P Image

GTX630654 WB Image

GTX630654 IHC-P Image

文献引用

Application Reference

产品评论

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