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Glypican-3抗体 [GT2473]

Orthogonal
Anti-Glypican-3 antibody [GT2473] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633410
Anti-Glypican-3 antibody [GT2473] used in IHC (Paraffin sections) (IHC-P). GTX633410
Anti-Glypican-3 antibody [GT2473] used in IHC (Paraffin sections) (IHC-P). GTX633410
Anti-Glypican-3 antibody [GT2473] used in Western Blot (WB). GTX633410
Anti-Glypican-3 antibody [GT2473] used in Western Blot (WB). GTX633410

目录号 GTX633410

文献引用 1
1

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT2473

同种型

 IgG1

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品说明

摘要

Glypican-3 (GPC3) is a glycoprotein attached to the cell membrane. It has a predicted molecular weight of 66 kDa. GPC3 overexpression is found in human hepatocellular carcinoma (HCC), but not in healthy adult liver. Studies have shown GPC3 to be a reliable serological and immunohistochemical marker for HCC diagnosis, and a potential target for immunotherapies.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

66 kDa. ( Note )

预测种属反应

Bovine, Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human Glypican-3. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888342

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

glypican 3 , DGSX , GTR2-2 , MXR7 , OCI-5 , SDYS , SGB , SGBS , SGBS1

细胞定位

Cell membrane; Lipid-anchor , GPI-anchor; Extracellular side , Secreted glypican-3: Secreted , extracellular space

功能与背景

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Glypican-3 antibody [GT2473] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633410

GTX633410 ICC/IF Image

Glypican-3 antibody [GT2473] detects Glypican-3 protein at cell membrane by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glypican-3 protein stained by Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:250.
Blue: Hoechst 33342 staining.
Anti-Glypican-3 antibody [GT2473] used in IHC (Paraffin sections) (IHC-P). GTX633410

GTX633410 IHC-P Image

Glypican-3 antibody [GT2473] detects Glypican-3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreatic cancer.
Glypican-3 stained by Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glypican-3 antibody [GT2473] used in IHC (Paraffin sections) (IHC-P). GTX633410

GTX633410 IHC-P Image

Glypican-3 antibody [GT2473] detects Glypican-3 protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human liver cancer.
Glypican-3 stained by Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:100.
Antigen Retrieval: Citrate buffer, 20 min

Anti-Glypican-3 antibody [GT2473] used in Western Blot (WB). GTX633410

GTX633410 WB Image

Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Glypican-3 antibody [GT2473] used in Western Blot (WB). GTX633410

GTX633410 WB Image

Orthogonal

Unboiled various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican-3 antibody [GT2473] (GTX633410) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

文献引用

Application Reference

Xue Y et al. Am J Pathol 2018; (Epub) Hepatitis C Virus Mimics Effects of Glypican-3 on CD81 and Promotes Development of Hepatocellular Carcinomas via Activation of Hippo Pathway in Hepatocytes.
实验应用 : WB
种属反应 : Human
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产品评论

Glypican-3 antibody [GT2473] 目录号 GTX633410

Rating
( Average 5 based on 1 users reviews)
实验应用
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-Glypican-3 antibody [GT2473] used in IHC (Paraffin sections) (IHC-P). GTX633410
Date : Anonymous submitted on 21-Oct-2020
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : liver cancer tissue
Fixation : Citrate buffer, 0.3°C
Primary Antibody : 1:100, 4°C, 16Hr
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SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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