产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000-1:10000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
human HMGCS2-transfected 293T cells
预测种属反应
Mouse, Rat, Bovine(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human HMGCS2. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2885675
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
3-hydroxy-3-methylglutaryl-CoA synthase 2
细胞定位
Mitochondrion
功能与背景
Mitochondrial 3-hydroxy-3-methylglutaryl CoA synthase (HMGCS2; EC 2.3.3.10) mediates the first reaction of ketogenesis, a metabolic pathway that provides lipid-derived energy for brain, heart, kidney, and other organs during times of carbohydrate deprivation such as fasting (Robinson and Williamson, 1980 [PubMed 6986618]). Also see cytoplasmic HMG-CoA synthase (HMGCS1; MIM 142940), which mediates an early step in cholesterol synthesis.[supplied by OMIM]
数据库
研究领域
產品图片
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GTX128013 WB Image
HMGCS2 antibody detects HMGCS2 protein by Western blot analysis.
A. 30 ug 293T whole cell lysate/extract
B. 30 whole cell lysate/extract of human HMGCS2-transfected 293T cells
7.5 % SDS-PAGE
HMGCS2 antibody (GTX128013) dilution: 1:5000
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GTX128013 IHC-P Image
HMGCS2 antibody detects HMGCS2 protein at mitochondria on human liver carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human liver carcinoma.
HMGCS2 antibody (GTX128013) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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