产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
HCT116 (UVB treatment 50mJ for 2hr) , HCT116 (UVB treatment 50mJ for 6hr) , NIH-3T3 (30μM cisplatin treatment for 24hr) , HCT-116 (30 uM cisplatin treatment for 24 hr)
预测种属反应
Bovine, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.16 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide surrounding phospho Ser139 of human Histone H2A.X. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2885642
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
,H2A histone family member X,gamma H2A.X,gamma H2AX,H2A.X,H2A/X,H2AX,H2AFX,
细胞定位
Nucleus
功能与背景
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX127340 WB Image
Histone H2A.X (phospho Ser139) antibody detects H2AFX protein in cisplatin-treated samples by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract (untreated)
B. 30 μg NIH-3T3 whole cell lysate/extract (30μM cisplatin treatment for 24hr)
15% SDS-PAGE
Histone H2A.X (phospho Ser139) antibody (GTX127340) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX127340 WB Image
Histone H2A.X (phospho Ser139) antibody detects H2AFX protein by western blot analysis.
A. 30 μg HCT116 whole cell lysate/extract (untreated for 2hr)
B. 30 μg HCT116 whole cell lysate/extract (UVB treatment 50mJ for 2hr)
C. 30 μg HCT116 whole cell lysate/extract (untreated for 6hr)
D. 30 μg HCT116 whole cell lysate/extract (UVB treatment 50mJ for 6hr)
15% SDS-PAGE
Histone H2A.X (phospho Ser139) antibody (GTX127340) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX127340 ICC/IF Image
Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H2A.XS139ph (phospho Ser139) stained by Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 μm.
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GTX127340 WB Image
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX127340 IHC-P Image
Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Histone H2A.XS139ph (phospho Ser139) stained by Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX127340 WB Image
Untreated (–) and treated (+) PC-12 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX127340 WB Image
Untreated (–) and treated (+) HCT-116 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX127340 ICC/IF Image
The data was published in the 2022 in Cell Death Dis. PMID: 35688824
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GTX127340 ICC/IF Image
The data was published in the journal FEBS Open Bio in 2018. PMID: 29928579
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GTX127340 WB Image
The data was published in the journal Cell Death Discov in 2016. PMID: 27551500
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GTX127340 WB Image
The data was published in the journal Cell Death Discov in 2016. PMID: 27551500
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文献引用
产品评论
Histone H2A.XS139ph (phospho Ser139) antibody
目录号 GTX127340
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Rating
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( Average 5 based on 2 users reviews)
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实验应用
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Western Blot(WB)
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( Average 5 based on 2 users reviews)
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Date :
Anonymous submitted on 23-Jun-2020
Application Tested :
WB
Sample Species :
Hu
Sample :
HeLa cells
Amount used :
20 μg
Blocking :
5% non-fat milk in TBST, 22°C, 0.5Hr
Primary Antibody :
1:3000, 4°C, 16Hr
Date :
Anonymous submitted on 15-Sep-2014
Application Tested :
WB
Sample Species :
Hu
Sample :
M10 cells
Amount used :
30μg
Blocking :
0.05% not fat milk, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
