Histone H3K27me3 (Tri-methyl Lys27)抗体

Name: Histone H3K27me3 (Tri-methyl Lys27)抗体
Brand: GeneTex
SKU: GTX121184
Price: 4000.00 RMB
Rating: 4.3 (3 reviews)

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX121184

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Western Blot (WB). GTX121184

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in IHC (Frozen sections) (IHC-Fr). GTX121184

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Dot blot (Dot). GTX121184

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121184

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in IHC (Whole mount) (IHC-Wm). GTX121184

目录号 GTX121184

文献引用 11

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, Dot, EM
种属反应	Human, Mouse, Rat, Zebrafish
产品包装	100 μl (¥4000), 25 μl (¥1700)

查看所有 Histone H3 的产品

View product citations for antibody GTX121184 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000
IHC-Wm	1:100-1:500
Dot	Assay dependent
EM	Assay dependent

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

阳性对照

Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

预测种属反应

Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide surrounding tri-methyl Lys27 of human Histone H3. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10618572

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

研究领域

產品图片

GTX121184 IHC-P Image

Histone H3K27me3 (trimethyl Lys27) antibody detects Histone H3K27me3 (trimethyl Lys27) protein at nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Histone H3K27me3 (trimethyl Lys27) antibody (GTX121184) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX121184 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K27me3 (Tri-methyl Lys27) antibody (GTX121184) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX121184 IHC-Fr Image

Immunofluorescence photomicrographs of frozen sections of mouse liver.
Red: Histone H3K27me3 (trimethyl Lys27) antibody (GTX121184) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight594) (GTX213110-05).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX121184 Dot Image

Various peptide samples were spotted onto positively charged nylon membrane, and blotted with Histone H3 (tri-Methyl Lys27) antibody (GTX121184) diluted at 1:2500.

GTX121184 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K27me3 (trimethyl Lys27) antibody (GTX121184) diluted at 1:1000.

GTX121184 IHC-P Image

Histone H3K27me3 (Tri-methyl Lys27) antibody detects Histone H3K27me3 (Tri-methyl Lys27) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Histone H3K27me3 (Tri-methyl Lys27) stained by Histone H3K27me3 (Tri-methyl Lys27) antibody (GTX121184) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX121184 IHC-P Image

Histone H3K27me3 (trimethyl Lys27) antibody detects Histone H3K27me3 (trimethyl Lys27) protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H3K27me3 (trimethyl Lys27) antibody (GTX121184) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX121184 ICC/IF Image

Histone H3K27me3 (Tri-methyl Lys27) antibody detects Histone H3K27me3 (Tri-methyl Lys27) protein at nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3K27me3 (Tri-methyl Lys27) stained by Histone H3K27me3 (Tri-methyl Lys27) antibody (GTX121184) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

GTX121184 IHC-Wm Image

Histone H3K27me3 (trimethyl Lys27) antibody detects Histone H3K27me3 (trimethyl Lys27) protein on zebrafish by whole mount immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
Histone H3K27me3 (trimethyl Lys27) antibody (GTX121184) dilution: 1:100.

GTX121184 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using Histon H3 (tri-Methyl K27)(GTX121184) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX121184 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using Histone H3 (tri-Methyl K27)(GTX121184) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX121184 IHC-P Image

GTX121184 WB Image

U87 cells were grown under normoxic (DMEM 10% FBS, 16% O2), hypoxic (DMEM 10% FBS, 1% O2), and neurosphere conditions (DMEM/F12, B-27 supplement, growth factor (10ng/ml FGF and 20ng/ml EGF)). Cell lysate were Western blotted for H3K27me3 and Histone-H3 (loading control). The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX121184 WB Image

文献引用

Application Reference

C Casali et al. Eur J Cell Biol 2023; 103 (1) : 151373 Impact of heat and cold shock on epigenetics and chromatin structure

实验应用 : EM

Perrigue PM et al. Cancers (Basel) 2020; 12 (7) Cancer Stem Cell-Inducing Media Activates Senescence Reprogramming in Fibroblasts.

Zhang T et al. Int J Nanomedicine 2020; 15 : 1457-1468 Anticancer Effects of Zinc Oxide Nanoparticles Through Altering the Methylation Status of Histone on Bladder Cancer Cells.

实验应用 : WB

种属反应 : Human

Hasebe T et al. Gen Comp Endocrinol 2020; 292 : 113441 Stem cell development involves divergent thyroid hormone receptor subtype expression and epigenetic modifications in the Xenopus metamorphosing intestine.

实验应用 : IHC-P

种属反应 : Human

Tanr?kulu B et al. Childs Nerv Syst 2020; (Epub) Is H3K27me3 status really a strong prognostic indicator for pediatric posterior fossa ependymomas? A single surgeon, single center experience.

实验应用 : IHC-P

种属反应 : Human

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我要回报文献

产品评论

Histone H3K27me3 (Tri-methyl Lys27) antibody 目录号 GTX121184

Rating

( Average 4.3 based on 3 users reviews)

实验应用

Western Blot(WB)		( Average 4 based on 2 users reviews)
Electron microscopy(EM)		( Average 5 based on 1 users reviews)

Date : Anonymous submitted on 26-May-2018

Score :

Application Tested : WB

Sample Species : Hu

Sample : MG63 cells

Amount used : 30 μg

Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr

Primary Antibody : 1:100, 4°C, 16Hr

条带说明 : Lane 1: MG63 whole cell lysate, Lane 2: doxorubicin resistant MG63 cell line, Lane 3: Cisplatin resistant MG63 cell line

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Electron microscopy (EM). GTX121184

Date : Anonymous submitted on 25-Jul-2016

Score :

Application Tested : EM

Sample Species : Rat

Sample : liver tissue

Blocking : Normal Goat Serum in PBS, 25°C, 0.08Hr

Fixation : 4% PFA in S?rensen phosphate buffer (pH 7.2)

Permeabilization /Antigen retrieval : 0.1% BSA in PBST

Primary Antibody : 1:50, 4°C, 16Hr

Date : Anonymous submitted on 20-Jan-2014

Score :

Application Tested : WB

Sample Species : Hu

Sample : 293T cells

Amount used : 60μg

Blocking : 5% non-fat milk, 25°C, 1Hr

Primary Antibody : 1:1000, 4°C, 16Hr

我要评论产品

Application Reference

C Casali et al. Eur J Cell Biol 2023; 103 (1):151373 Impact of heat and cold shock on epigenetics and chromatin structure

实验应用 : EM

Perrigue PM et al. Cancers (Basel) 2020; 12 (7) Cancer Stem Cell-Inducing Media Activates Senescence Reprogramming in Fibroblasts.

Zhang T et al. Int J Nanomedicine 2020; 15:1457-1468 Anticancer Effects of Zinc Oxide Nanoparticles Through Altering the Methylation Status of Histone on Bladder Cancer Cells.

实验应用 : WB

种属反应 : Human

Hasebe T et al. Gen Comp Endocrinol 2020; 292:113441 Stem cell development involves divergent thyroid hormone receptor subtype expression and epigenetic modifications in the Xenopus metamorphosing intestine.

实验应用 : IHC-P

种属反应 : Human

Tanr?kulu B et al. Childs Nerv Syst 2020; (Epub) Is H3K27me3 status really a strong prognostic indicator for pediatric posterior fossa ependymomas? A single surgeon, single center experience.

实验应用 : IHC-P

种属反应 : Human

Blin G et al. Nutrients 2020; 12 (1) Maternal Exposure to High-Fat Diet Induces Long-Term Derepressive Chromatin Marks in the Heart.

实验应用 : WB

种属反应 : Rat

Tsuyama N et al. Hematol Oncol 2017; Epstein-Barr virus-negative extranodal "true" natural killer-cell lymphoma harbouring a KDM6A mutation.

实验应用 : IHC-P

种属反应 : Human

Mirzamohammadi F et al. Nat Commun 2016; 7:12047 Polycomb repressive complex 2 regulates skeletal growth by suppressing Wnt and TGF-β signalling.

实验应用 : WB

种属反应 : Mouse

Perrigue PM et al. Mol Cancer Res 2015; 13 (4):636-50 The histone demethylase jumonji coordinates cellular senescence including secretion of neural stem cell-attracting cytokines.

Liu TP et al. Anticancer Drugs 2015; 26 (2):139-47 S-Adenosyl-L-methionine-competitive inhibitors of the histone methyltransferase EZH2 induce autophagy and enhance drug sensitivity in cancer cells.

实验应用 : WB

种属反应 : Human

Jiang X et al. FASEB J 2012; 26 (8):3563-74 Maternal choline intake alters the epigenetic state of fetal cortisol-regulating genes in humans.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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	http://www.neobioscience.com
	genetex@neobioscience.com

Histone H3K27me3 (Tri-methyl Lys27)抗体

目录号 GTX121184

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

阳性对照

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

研究领域

產品图片

GTX121184 IHC-P Image

GTX121184 WB Image

GTX121184 IHC-Fr Image

GTX121184 Dot Image

GTX121184 WB Image

GTX121184 IHC-P Image

GTX121184 IHC-P Image

GTX121184 ICC/IF Image

GTX121184 IHC-Wm Image

GTX121184 IHC-P Image

GTX121184 ICC/IF Image

GTX121184 ICC/IF Image

GTX121184 IHC-P Image

GTX121184 WB Image

GTX121184 WB Image

GTX121184 WB Image

文献引用

Application Reference

产品评论

Histone H3K27me3 (Tri-methyl Lys27) antibody 目录号 GTX121184

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