GeneTex中国官方网站
  • China

KLF4抗体

KO/KD Overexpression
Anti-KLF4 antibody used in Western Blot (WB). GTX101509
Anti-KLF4 antibody used in Western Blot (WB). GTX101509
Anti-KLF4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101509
Anti-KLF4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101509
Anti-KLF4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101509

目录号 GTX101509

文献引用 7
2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 KLF4 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

55 kDa. ( Note )

阳性对照

HeLa , KLF4-transfected 293T

预测种属反应

Rat, Sheep, Bovine, Cat, Pig, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human KLF4. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11173878

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Kruppel like factor 4 , EZF , GKLF

细胞定位

Nucleus

功能与背景

Transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.

数据库

研究领域

產品图片

Anti-KLF4 antibody used in Western Blot (WB). GTX101509

GTX101509 WB Image

KO/KD

Wild-type (WT) and KLF4 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KLF4 antibody (GTX101509) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF4 antibody used in Western Blot (WB). GTX101509

GTX101509 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody (GTX101509) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101509

GTX101509 ICC/IF Image

KLF4 antibody detects KLF4 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: KLF4 protein stained by KLF4 antibody (GTX101509) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-KLF4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101509

GTX101509 IHC-P Image

KLF4 antibody detects KLF4 protein at nucleus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
KLF4 antibody (GTX101509) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-KLF4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101509

GTX101509 IHC-P Image

KLF4 antibody detects KLF4 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
KLF4 antibody (GTX101509) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

文献引用

Application Reference

Feng DD et al. Front Cardiovasc Med 2021; 8 : 768662 17β-Estradiol Inhibits Proliferation and Oxidative Stress in Vascular Smooth Muscle Cells by Upregulating BHLHE40 Expression.
实验应用 : WB
种属反应 : Mouse
Zhou J et al. J Ethnopharmacol 2020; 262 : 113208 Salvia miltiorrhiza bunge exerts anti-oxidative effects through inhibiting KLF10 expression in vascular smooth muscle cells exposed to high glucose.
实验应用 : WB
种属反应 : Mouse
Nakayama I et al. J Biochem 2020; (Epub) Regulation of Epidermal Growth Factor Receptor Expression and Morphology of Lung Epithelial Cells by Interleukin-1帣.
实验应用 : ICC/IF
种属反应 : Human
Takeuchi Y et al. Exp Cell Res 2018; 371 (2) : 417-425 Kruppel-Like Factor 4 represses osteoblast differentiation via ciliary Hedgehog signaling.
实验应用 : WB
种属反应 : Mouse
Moon BS et al. Nat Commun 2018; 9 (1) : 401 Kruppel-like factor 4-dependent Staufen1-mediated mRNA decay regulates cortical neurogenesis.
种属反应 : Mouse
显示更多 +
我要回报文献

产品评论

KLF4 antibody 目录号 GTX101509

Rating
( Average 3 based on 2 users reviews)
实验应用
Western Blot(WB)
( Average 3 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 3 based on 1 users reviews)
Anti-KLF4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101509
Date : Anonymous submitted on 10-Apr-2019
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : Mouse iPS cells
Blocking : 20% ImmunoBlock, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5% Triton X-100
Primary Antibody : 1:250, 4°C, 16Hr
Anti-KLF4 antibody used in Western Blot (WB). GTX101509
Date : Anonymous submitted on 15-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells, T24 cells
Amount used : 1* 10^5 cells
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
条带说明 : Lane 1: A549 cells, Lane 2: T24 cells.
我要评论产品
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com