产品说明
摘要
LDHA facilitates glycolytic flux by converting pyruvate to lactate and NADH to NAD+. It is primarily cytoplasmic, but is also found in mitochondria and the nucleus. In addition to its metabolic function, LDHA is also a single‐stranded DNA‐binding protein participating in DNA duplication and transcription. Elevated expression of LDHA is observed in various cancers.
产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
PC-12 , Rat2 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , 293T , A431 , HeLa , HepG2
预测种属反应
Rabbit, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.33 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human LDHA. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_10726413
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
lactate dehydrogenase A , GSD11 , HEL-S-133P , LDHM , PIG19
细胞定位
Cytoplasm
功能与背景
The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq]
数据库
研究领域
產品图片
|
GTX101416 IHC-P Image
LDHA antibody detects LDHA protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.LDHA stained by LDHA antibody (GTX101416) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX101416 WB Image
LDHA antibody detects LDHA protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg C8D30 whole cell lysate/extract
C. 30 μg NIH-3T3 whole cell lysate/extract
D. 30 μg Raw264.7 whole cell lysate/extract
E. 30 μg C2C12 whole cell lysate/extract
10% SDS-PAGE
LDHA antibody (GTX101416) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX101416 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human lung papillory adenocarcinoma, using LDHA(GTX101416) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
|
GTX101416 ICC/IF Image
Immunofluorescence analysis of methanol-fixed A431, using LDHA(GTX101416) antibody at 1:500 dilution.
|
|
|
GTX101416 ICC/IF Image
LDHA antibody detects LDHA protein at cytoplasm by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LDHA protein stained by LDHA antibody (GTX101416) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
|
|
|
GTX101416 WB Image
LDHA antibody detects LDHA protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
10% SDS-PAGE
LDHA antibody (GTX101416) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX101416 ICC/IF Image
LDHA antibody detects LDHA protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LDHA protein stained by LDHA antibody (GTX101416) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
|
|
|
GTX101416 WB Image
LDHA antibody detects LDHA protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDHA antibody (GTX101416) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX101416 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with LDHA antibody (GTX101416) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX101416 WB Image
The data was published in the journal PLoS One in 2017. PMID: 29045486
|
|
|
GTX101416 WB Image
The data was published in the journal PLoS One in 2017. PMID: 29045486
|
|
|
GTX101416 WB Image
The data was published in the journal Cell Death Dis in 2017. PMID: 28055010
|
|
|
GTX101416 WB Image
The data was published in the 2022 in Int J Mol Sci. PMID: 35328751
|
文献引用
产品评论
LDHA antibody
目录号 GTX101416
|
Rating
|
|
( Average 5 based on 1 users reviews)
|
实验应用
|
Western Blot(WB)
|
|
( Average 5 based on 1 users reviews)
|
Date :
Anonymous submitted on 29-May-2018
Application Tested :
WB
Sample Species :
Hu
Sample :
MCF7 cells
Blocking :
5%milk in TBST
Primary Antibody :
1:1000, 4°C, 16Hr
