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Lac I抗体

Anti-Lac I antibody used in Western Blot (WB). GTX48707

目录号 GTX48707

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, IP, ELISA

种属反应

E. coli, Shigella sonnei
产品包装
50 μg (¥5300)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:2000
IP Assay dependent
ELISA 1:90000-1:100000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品属性

形式

Liquid

存储溶液

20mM Potassium Phosphate, 150mM NaCl

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

E. coli

免疫原

Full length recombinant Lac I protein.

纯化方式

Purified by antigen-affinity chromatography.
From serum

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

功能与背景

The lac repressor protein (Lac I) is an important genetic control protein. It controls the expression of the lactose metabolizing enzymes in Escherichia coli. This regulation involves specific recognition of the operator DNA sequence (Lac O) and consequent inhibition of transcription initiation by RNA polymerase; modulation of binding at this target site by small sugar molecules, inducers, provides the basis for regulation. Different ligand binding activities are associated with the isolated domains of Lac I: specific and nonspecific DNA binding with the NH2 termini, and inducer and specific DNA binding with the core protein. This antibody could possibly be used to recover and purify protein/nucleic acid complexes (host and viral proteins associated with the viral DNA) from infected cells.

研究领域

產品图片

Anti-Lac I antibody used in Western Blot (WB). GTX48707

GTX48707 WB Image

Western blot using GeneTex's affinity purified anti-Lac I antibody shows detection of a 38 kDa band corresponding to recombinant Lac I (arrowhead). The blot was blocked with 5% BLOTTO in TBST overnight at 4ºC. Primary antibody was used at a 1:1,000 dilution followed by reaction with a 1:10,000 dilution of HRP goat anti-rabbit IgG. ECL was used for detection.

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SDS
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 5300
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