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Mitofilin抗体

Anti-Mitofilin antibody used in Western Blot (WB). GTX115523
Anti-Mitofilin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115523

目录号 GTX115523

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IP

种属反应

Human
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

84 kDa. ( Note )

阳性对照

U87-MG

预测种属反应

Mouse(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human Mitofilin. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11166340

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

inner membrane mitochondrial protein , HMP , MICOS60 , MINOS2 , Mic60 , P87 , P87/89 , P89 , PIG4 , PIG52

细胞定位

Mitochondrion inner membrane

数据库

研究领域

產品图片

Anti-Mitofilin antibody used in Western Blot (WB). GTX115523

GTX115523 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Mitofilin antibody (GTX115523) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Mitofilin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115523

GTX115523 ICC/IF Image

Mitofilin antibody detects Mitofilin protein at mitochondria by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: Mitofilin protein stained by Mitofilin antibody (GTX115523) diluted at 1:500.
Blue: Hoechst 33342 staining.

文献引用

产品评论

Mitofilin antibody 目录号 GTX115523

Rating ( Average 5 based on 1 users reviews)
实验应用
Immunoprecipitation(IP) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 28-Jul-2016
Score :
Application Tested : IP
Sample Species : Hu
Sample : MM.1S cells
Amount used : 300μg for IP
Blocking : 5% milk in TBS, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : Immunoprecipitation of Mitofilin protein from MM.1S whole cell extracts using 3 μg of Mitofilin antibody (GTX115523) and detected by Mitofilin antibody of WB.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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