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NIH-3T3 whole 细胞裂解物

NIH-3T3 whole cell lysate. GTX27901

目录号 GTX27901

Speciess

 Mouse
产品包装
100 μg (¥2000)
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产品说明

摘要

NIH-3T3 embryonic cells grown to 85% confluency were harvested, homogenized in modified RIPA buffer (150 mM NaCl, 50 mM Tris-Cl, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, 1 mM EDTA, 1 mM PMSF, 5 μg/ml Pepstatin A, 2 μg/ml Leupeptin, 1 mM sodium orthovanadate, 1 mM sodium fluoride.), and cleared by centrifugation. Total protein concentration of the lysate was determined using BCA protein assay (BSA was used as a standard). Lysate was mixed with Laemmli Sample Buffer containing reducing reagent and was boiled for 5 minutes. Lysate is supplied as 200 μg in 100 μl per vial (2.0 mg/ml total protein concentration) in 1X Laemmli sample buffer.

产品属性

形式

Liquid

存储溶液

1x SDS sample buffer, 5% β-mercaptoethanol

保存剂

No preservative

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

纯化方式

Whole Cell Lysate

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

產品图片

NIH-3T3 whole cell lysate. GTX27901

GTX27901 Image

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产品包装 报价 (¥)
¥ 2000
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