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NRF2抗体 [HL1021]

Recombinant antibody Comparison Orthogonal Overexpression
Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826
Anti-NRF2 antibody [HL1021] used in IHC (Paraffin sections) (IHC-P). GTX635826
Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826
Anti-NRF2 antibody [HL1021] used in IHC (Paraffin sections) (IHC-P). GTX635826
Anti-NRF2 antibody [HL1021] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635826
Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826
Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826
Anti-NRF2 antibody [HL1021] used in IHC (Paraffin sections) (IHC-P). GTX635826
Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826

目录号 GTX635826

文献引用 5

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1021

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, PLA

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
PLA Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

68 kDa. ( Note )

预测种属反应

Bovine, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human NRF2. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2909905

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

nuclear factor, erythroid 2 like 2 , HEBP1 , IMDDHH , NRF2

细胞定位

Cytoplasm, cytosol,Nucleus

功能与背景

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015]

数据库

研究领域

產品图片

Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826

GTX635826 WB Image

Comparison Orthogonal

Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with NRF2 antibody [HL1021] (GTX635826) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-NRF2 antibody [HL1021] used in IHC (Paraffin sections) (IHC-P). GTX635826

GTX635826 IHC-P Image

NRF2 antibody [HL1021] detects NRF2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissue.
NRF2 stained by NRF2 antibody [HL1021] (GTX635826) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826

GTX635826 WB Image

Orthogonal

Untreated (–) and treated (+) Rat2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [HL1021] (GTX635826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NRF2 antibody [HL1021] used in IHC (Paraffin sections) (IHC-P). GTX635826

GTX635826 IHC-P Image

NRF2 antibody [HL1021] detects NRF2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
NRF2 stained by NRF2 antibody [HL1021] (GTX635826) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NRF2 antibody [HL1021] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635826

GTX635826 ICC/IF Image

Orthogonal

NRF2 antibody [HL1021] detects NRF2 protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NRF2 stained by NRF2 antibody [HL1021] (GTX635826) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826

GTX635826 WB Image

Orthogonal

Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [HL1021] (GTX635826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826

GTX635826 WB Image

Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [HL1021] (GTX635826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NRF2 antibody [HL1021] used in IHC (Paraffin sections) (IHC-P). GTX635826

GTX635826 IHC-P Image

NRF2 antibody [HL1021] detects NRF2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse salivary gland.
NRF2 stained by NRF2 antibody [HL1021] (GTX635826) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NRF2 antibody [HL1021] used in Western Blot (WB). GTX635826

GTX635826 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [HL1021] (GTX635826) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Huang YX et al. Int J Mol Sci 2024; 25 (4) Lysophosphatidic Acid Receptor 3 Activation Is Involved in the Regulation of Ferroptosis.
Zhang W et al. Oxid Med Cell Longev 2023; 2023 : 9966355 Tubeimoside I Ameliorates Doxorubicin-Induced Cardiotoxicity by Upregulating SIRT3.
实验应用 : WB
种属反应 : Mouse, Rat
Feng C et al. Cells 2022; 11 (3) Static Magnetic Fields Reduce Oxidative Stress to Improve Wound Healing and Alleviate Diabetic Complications.
实验应用 : WB
种属反应 : Mouse
Lin CY et al. Antioxidant 2021; 10 (12) : 1898 Glucose Activates Lysine-Specific Demethylase 1 through the KEAP1/p62 Pathway.
实验应用 : PLA
种属反应 : Human
Eslami M et al. Cell J 2021; 23 (3) : 319-328 Astaxanthin Protects Human Granulosa Cells against Oxidative Stress through Activation of NRF2/ARE Pathway and Its Downstream Phase II Enzymes.
实验应用 : WB
种属反应 : Human
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