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Nestin抗体 [GT623]

Comparison Orthogonal
Anti-Nestin antibody [GT623] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630201
Anti-Nestin antibody [GT623] used in Western Blot (WB). GTX630201
Anti-Nestin antibody [GT623] used in Western Blot (WB). GTX630201

目录号 GTX630201

文献引用 12

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT623

同种型

 IgG1

实验应用

 WB, ICC/IF

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品说明

摘要

Nestin antibody recognizes nestin, a type-IV intermediate filament protein that migrates at ~240 kDa on western blot. Nestin is an established marker of mesodermal and neuroectodermal fates, and is expressed in neural stem cells. It is also enriched in endothelial cells, regardless of their proliferative state.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

177 kDa. ( Note )

阳性对照

SK-N-SH , IMR32

产品说明

我们不建议将此产品应用于 Mouse,Rat samples.

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human Nestin. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888203

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

nestin , Nbla00170

功能与背景

Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]

数据库

研究领域

產品图片

Anti-Nestin antibody [GT623] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630201

GTX630201 ICC/IF Image

Nestin antibody [GT623] detects Nestin protein at cytoskeleton by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in ice-cold MeOH for 5 min.
Green: Nestin stained by Nestin antibody [GT623] (GTX630201) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Nestin antibody [GT623] used in Western Blot (WB). GTX630201

GTX630201 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nestin antibody [GT623] (GTX630201) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Nestin antibody [GT623] used in Western Blot (WB). GTX630201

GTX630201 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with Nestin antibody [GT623] (GTX630201) diluted at 1:1000 and competitor's antibody (MAB5326) diluted by 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

文献引用

Application Reference

S Sakatoku et al. Stem Cells Int 2024; : 7685280 Periostin Is a Candidate Regulator of the Host Microenvironment in Regeneration of Pulp and Dentin Complex and Periodontal Ligament in Transplantation with Stem Cell-Conditioned Medium
实验应用 : ICC/IF
Seo Y et al. Int J Mol Sci 2023; 24 (6) Endogenous Neural Stem Cell Activation after Low-Intensity Focused Ultrasound-Induced Blood-Brain Barrier Modulation.
实验应用 : WB
Yao P et al. Folia Histochem Cytobiol 2022; (Epub) Wnt/PCP pathway regulates the migration and neural differentiation of mesenchymal stem cells in vitro.
Ma L et al. J Radiat Res 2022; (Epub) Irradiated microvascular endothelial cells may induce bystander effects in neural stem cells leading to neurogenesis inhibition.
Ming Zhang et al. Nat Commun 2021; 12 (1) : 5091 Ten-eleven translocation 1 mediated-DNA hydroxymethylation is required for myelination and remyelination in the mouse brain.
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Application Reference
S Sakatoku et al. Stem Cells Int 2024;:7685280 Periostin Is a Candidate Regulator of the Host Microenvironment in Regeneration of Pulp and Dentin Complex and Periodontal Ligament in Transplantation with Stem Cell-Conditioned Medium
实验应用 : ICC/IF
Seo Y et al. Int J Mol Sci 2023; 24 (6) Endogenous Neural Stem Cell Activation after Low-Intensity Focused Ultrasound-Induced Blood-Brain Barrier Modulation.
实验应用 : WB
Yao P et al. Folia Histochem Cytobiol 2022; (Epub) Wnt/PCP pathway regulates the migration and neural differentiation of mesenchymal stem cells in vitro.
Ma L et al. J Radiat Res 2022; (Epub) Irradiated microvascular endothelial cells may induce bystander effects in neural stem cells leading to neurogenesis inhibition.
Ming Zhang et al. Nat Commun 2021; 12 (1):5091 Ten-eleven translocation 1 mediated-DNA hydroxymethylation is required for myelination and remyelination in the mouse brain.
Deng H et al. Cell Transplant 2020; 29:9.6369E+14 Long-Term Liraglutide Administration Induces Pancreas Neogenesis in Adult T2DM Mice.
Koffler J et al. Nat Med 2019; 25 (2):263-269 Biomimetic 3D-printed scaffolds for spinal cord injury repair.
Mansel C et al. Neurotoxicol Teratol 2019; 72:58-70 Lead exposure reduces survival, neuronal determination, and differentiation of P19 stem cells.
Liu H et al. Cell Death Differ 2018; (Epub) Biochemical re-programming of human dermal stem cells to neurons by increasing mitochondrial membrane potential.
种属反应 : Human
Xie MQ et al. Neuroscience 2017; 352:64-78 Newborn dopaminergic neurons are associated with the migration and differentiation of SVZ-derived neural progenitors in a 6-hydroxydopamin-injected mouse model.
Aguilera-Castrejon A et al. Neurochem Res 2016; (Epub) Improved Proliferative Capacity of NP-Like Cells Derived from Human Mesenchymal Stromal Cells and Neuronal Transdifferentiation by Small Molecules.
种属反应 : Human
Pringproa K et al. PLoS One 2015; 10 (8):e0135850 Tropism and Induction of Cytokines in Human Embryonic-Stem Cells-Derived Neural Progenitors upon Inoculation with Highly- Pathogenic Avian H5N1 Influenza Virus.
种属反应 : Human
SDS
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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