产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
mouse brain , PC-12
预测种属反应
Bovine, Pig, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human O-GlcNAc transferase. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein G.
偶联
Unconjugated
RRID
AB_2888160
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
O-linked N-acetylglucosamine (GlcNAc) transferase , HINCUT-1 , HRNT1 , MRX106 , O-GLCNAC , OGT1
细胞定位
Cytoplasm (Potential) , Nucleus
功能与背景
O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]
数据库
研究领域
產品图片
![Anti-O-GlcNAc transferase antibody [GT678] used in Western Blot (WB). GTX629812](/templates/default/js/lightbox/images/loading.gif "Anti-O-GlcNAc transferase antibody [GT678] used in Western Blot (WB). GTX629812")
|
GTX629812 WB Image
O-GlcNAc transferase antibody [GT6G8] detects O-GlcNAc transferase protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
7.5% SDS-PAGE
O-GlcNAc transferase antibody [GT6G8] (GTX629812) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
|
|
GTX629812 WB Image
O-GlcNAc transferase antibody [GT6G8] detects O-GlcNAc transferase protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
7.5% SDS-PAGE
O-GlcNAc transferase antibody [GT6G8] (GTX629812) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
|
|
GTX629812 IHC-P Image
O-GlcNAc transferase antibody [GT678] detects O-GlcNAc transferase protein at cytoplasm of islet by immunohistochemical analysis.Sample: Paraffin-embedded mouse pancreas.O-GlcNAc transferase stained by O-GlcNAc transferase antibody [GT678] (GTX629812) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX629812 ICC/IF Image
O-GlcNAc transferase antibody [GT678] detects O-GlcNAc transferase protein at nucleus (mainly) and cytoplasm (less) by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: O-GlcNAc transferase protein stained by O-GlcNAc transferase antibody [GT678] (GTX629812) diluted at 1:500.
Blue: Hoechst 33342 staining.
|
文献引用
目前没有使用 O-GlcNAc transferase antibody [GT678] (GTX629812) 的文献被收录。快来回报我们第一篇参考文献吧！
产品评论
目前没有使用 O-GlcNAc transferase antibody [GT678] (GTX629812) 的产品评论被收录。快发表第一篇产品评论吧！
![Anti-O-GlcNAc transferase antibody [GT678] used in Western Blot (WB). GTX629812](/upload/website/prouct_img/small/GTX629812/GTX629812_41477_WB_M_brain_w_23061202_902.webp "Anti-O-GlcNAc transferase antibody [GT678] used in Western Blot (WB). GTX629812")
![Anti-O-GlcNAc transferase antibody [GT678] used in Western Blot (WB). GTX629812](/upload/website/prouct_img/small/GTX629812/GTX629812_41477_WB_R_w_23061202_572.webp "Anti-O-GlcNAc transferase antibody [GT678] used in Western Blot (WB). GTX629812")
![Anti-O-GlcNAc transferase antibody [GT678] used in IHC (Paraffin sections) (IHC-P). GTX629812](/upload/website/prouct_img/small/GTX629812/GTX629812_41477_20220401_IHC-P_M_w_23061202_444.webp "Anti-O-GlcNAc transferase antibody [GT678] used in IHC (Paraffin sections) (IHC-P). GTX629812")
![Anti-O-GlcNAc transferase antibody [GT678] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629812](/upload/website/prouct_img/small/GTX629812/GTX629812_41477_IFA_w_23061202_883.webp "Anti-O-GlcNAc transferase antibody [GT678] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629812")
![Anti-O-GlcNAc transferase antibody [GT678] used in Western Blot (WB). GTX629812](/upload/website/prouct_img/normal/GTX629812/GTX629812_41477_WB_M_brain_w_23061202_902.webp "Anti-O-GlcNAc transferase antibody [GT678] used in Western Blot (WB). GTX629812")
![Anti-O-GlcNAc transferase antibody [GT678] used in Western Blot (WB). GTX629812](/upload/website/prouct_img/normal/GTX629812/GTX629812_41477_WB_R_w_23061202_572.webp "Anti-O-GlcNAc transferase antibody [GT678] used in Western Blot (WB). GTX629812")
![Anti-O-GlcNAc transferase antibody [GT678] used in IHC (Paraffin sections) (IHC-P). GTX629812](/upload/website/prouct_img/normal/GTX629812/GTX629812_41477_20220401_IHC-P_M_w_23061202_444.webp "Anti-O-GlcNAc transferase antibody [GT678] used in IHC (Paraffin sections) (IHC-P). GTX629812")
![Anti-O-GlcNAc transferase antibody [GT678] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629812](/upload/website/prouct_img/normal/GTX629812/GTX629812_41477_IFA_w_23061202_883.webp "Anti-O-GlcNAc transferase antibody [GT678] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629812")
