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Oct4抗体

Anti-Oct4 antibody used in Western Blot (WB). GTX101497
Anti-Oct4 antibody used in Immunoprecipitation (IP). GTX101497
Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101497
Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101497
Anti-Oct4 antibody used in Flow cytometry (FACS). GTX101497
Anti-Oct4 antibody used in Western Blot (WB). GTX101497
Anti-Oct4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101497
Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101497
Anti-Oct4 antibody used in Western Blot (WB). GTX101497
Anti-Oct4 antibody used in Western Blot (WB). GTX101497
Anti-Oct4 antibody used in Western Blot (WB). GTX101497
Anti-Oct4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101497
Anti-Oct4 antibody used in Western Blot (WB). GTX101497
Anti-Oct4 antibody used in Western Blot (WB). GTX101497
Anti-Oct4 antibody used in Western Blot (WB). GTX101497
Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101497
Anti-Oct4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101497

目录号 GTX101497

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP

种属反应

Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:5000-1:100000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
FACS 1:50-1:200
IP 1:500-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

39 kDa. ( Note )

阳性对照

mouse ESC , human ESC , NT2D1

预测种属反应

Rabbit, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10618784

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

POU class 5 homeobox 1 , OCT3 , OCT4 , OTF-3 , OTF3 , OTF4 , Oct-3 , Oct-4

细胞定位

Nucleus

功能与背景

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Oct4 antibody used in Western Blot (WB). GTX101497

GTX101497 WB Image

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX101497) diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Oct4 antibody used in Immunoprecipitation (IP). GTX101497

GTX101497 IP Image

Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX101497) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX101497) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101497

GTX101497 ICC/IF Image

Oct4 antibody detects Oct4 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Oct4 protein stained by Oct4 antibody (GTX101497) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101497

GTX101497 ICC/IF Image

Immunofluorescence analysis of hESCs on CF1 feeders, using OCT3/4(GTX101497) antibody at 1:200 dilution.

Anti-Oct4 antibody used in Flow cytometry (FACS). GTX101497

GTX101497 FACS Image

Flow cytometry on human embryonic stem cells, staining with Oct4 (GTX101497)antibody at 1:100 dilution(purple) or rabbit IgG (black).

Anti-Oct4 antibody used in Western Blot (WB). GTX101497

GTX101497 WB Image

Sample (20 μg of whole cell lysate)
A: mouse ESC
12% SDS PAGE
GTX101497 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Oct4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101497

GTX101497 IHC-P Image

Oct4 antibody detects Oct4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Oct4 stained by Oct4 antibody (GTX101497) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101497

GTX101497 ICC/IF Image

Immunofluorescence analysis of mESCs on CF1 feeders, using OCT3/4(GTX101497) antibody at 1:200 dilution.

Anti-Oct4 antibody used in Western Blot (WB). GTX101497

GTX101497 WB Image

Oct4 antibody detects Oct4 protein by western blot analysis.
A. 30 μg human ESC whole cell extract
12% SDS-PAGE
Oct4 antibody (GTX101497) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Oct4 antibody used in Western Blot (WB). GTX101497

GTX101497 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX101497) diluted at 1:100000.

Anti-Oct4 antibody used in Western Blot (WB). GTX101497

GTX101497 WB Image

The data was published in the journal Cell Death Dis in 2015. PMID: 26136074

Anti-Oct4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101497

GTX101497 IHC-P Image

The data was published in the journal BMC Cancer in 2019. PMID: 31399076

Anti-Oct4 antibody used in Western Blot (WB). GTX101497

GTX101497 WB Image

The data was published in the 2019 in Cancers (Basel). PMID: 31861872

Anti-Oct4 antibody used in Western Blot (WB). GTX101497

GTX101497 WB Image

The data was published in the journal Oncotarget in 2016. PMID: 26992205

Anti-Oct4 antibody used in Western Blot (WB). GTX101497

GTX101497 WB Image

The data was published in the journal Oncotarget in 2016. PMID: 26992205

Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101497

GTX101497 ICC/IF Image

The data was published in the journal Oncotarget in 2020.PMID: 32256979

Anti-Oct4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101497

GTX101497 IHC-P Image

The data was published in the journal BMC Cancer in 2019. PMID: 31399076

文献引用

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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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