产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:5000-1:20000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is specific for human PARP1 protein, and it does not cross react with human PARP2 and PARP3 protein.
预测种属反应
Rat, Bovine(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.5 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of Human PARP. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
poly(ADP-ribose) polymerase 1 , ADPRT , ADPRT 1 , ADPRT1 , ARTD1 , PARP , PARP-1 , PPOL , pADPRT-1
细胞定位
Nucleus,Nucleolus
功能与背景
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
![Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804](/templates/default/js/lightbox/images/loading.gif "Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804")
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GTX636804 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [HL1364] (GTX636804) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636804 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with PARP antibody [HL1364] (GTX636804) diluted at 1:10000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX636804 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [HL1364] (GTX636804) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636804 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [HL1364] (GTX636804) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX636804 WB Image
Untreated (–) and treated (+) HCT-116 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [HL1364] (GTX636804) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636804 IHC-P Image
PARP antibody [HL1364] detects PARP protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse spleen.PARP stained by PARP antibody [HL1364] (GTX636804) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636804 ICC/IF Image
PARP antibody [HL1364] detects PARP protein by immunofluorescent analysis.Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PARP stained by PARP antibody [HL1364] (GTX636804) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636804 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [HL1364] (GTX636804) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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![Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804](/upload/website/prouct_img/small/GTX636804/GTX636804_44711_20220819_WB_shRNA_watermark_22082402_911.webp "Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804")
![Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804](/upload/website/prouct_img/small/GTX636804/GTX636804_44711_20220812_WB_competitor_watermark_22081423_386.webp "Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804")
![Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804](/upload/website/prouct_img/small/GTX636804/GTX636804_44711_20231006_WB_multiple_B_23102401_688.webp "Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804")
![Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804](/upload/website/prouct_img/small/GTX636804/GTX636804_T-44599_20220506_WB_M_w_23061202_245.webp "Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804")
![Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804](/upload/website/prouct_img/small/GTX636804/GTX636804_44711_20220617_WB_treatment_Cisplatin_22062121_177.webp "Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804")
![Anti-PARP antibody [HL1364] used in IHC (Paraffin sections) (IHC-P). GTX636804](/upload/website/prouct_img/small/GTX636804/GTX636804_44711_20221228_IHC-P_M_22122901_230.webp "Anti-PARP antibody [HL1364] used in IHC (Paraffin sections) (IHC-P). GTX636804")
![Anti-PARP antibody [HL1364] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636804](/upload/website/prouct_img/small/GTX636804/GTX636804_44711_20221230_ICC_IF_22122901_761.webp "Anti-PARP antibody [HL1364] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636804")
![Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804](/upload/website/prouct_img/small/GTX636804/GTX636804_44711_20230714_WB_B_23071822_796.webp "Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804")
![Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804](/upload/website/prouct_img/normal/GTX636804/GTX636804_44711_20220819_WB_shRNA_watermark_22082402_911.webp "Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804")
![Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804](/upload/website/prouct_img/normal/GTX636804/GTX636804_44711_20220812_WB_competitor_watermark_22081423_386.webp "Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804")
![Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804](/upload/website/prouct_img/normal/GTX636804/GTX636804_44711_20231006_WB_multiple_B_23102401_688.webp "Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804")
![Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804](/upload/website/prouct_img/normal/GTX636804/GTX636804_T-44599_20220506_WB_M_w_23061202_245.webp "Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804")
![Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804](/upload/website/prouct_img/normal/GTX636804/GTX636804_44711_20220617_WB_treatment_Cisplatin_22062121_177.webp "Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804")
![Anti-PARP antibody [HL1364] used in IHC (Paraffin sections) (IHC-P). GTX636804](/upload/website/prouct_img/normal/GTX636804/GTX636804_44711_20221228_IHC-P_M_22122901_230.webp "Anti-PARP antibody [HL1364] used in IHC (Paraffin sections) (IHC-P). GTX636804")
![Anti-PARP antibody [HL1364] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636804](/upload/website/prouct_img/normal/GTX636804/GTX636804_44711_20221230_ICC_IF_22122901_761.webp "Anti-PARP antibody [HL1364] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636804")
![Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804](/upload/website/prouct_img/normal/GTX636804/GTX636804_44711_20230714_WB_B_23071822_796.webp "Anti-PARP antibody [HL1364] used in Western Blot (WB). GTX636804")
