产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:10000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is specific for human PAX6 protein, and it does not cross react with human PAX2, PAX4, PAX5, and PAX8 protein.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of Human PAX6.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
paired box 6 , AN , AN2 , ASGD5 , D11S812E , FVH1 , MGDA , WAGR
细胞定位
Nucleus
功能与背景
This gene encodes a homeobox and paired domain-containing protein that binds DNA and functions as a regulator of transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. This gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Mutations in this gene or in the enhancer regions can cause ocular disorders such as aniridia and Peter's anomaly. Use of alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015]
数据库
研究领域
產品图片
![Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051](/templates/default/js/lightbox/images/loading.gif "Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051")
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GTX639051 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody [HL2599] (GTX639051) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX639051 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody [HL2599] (GTX639051) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX639051 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody [HL2599] (GTX639051) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX639051 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody [HL2599] (GTX639051) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX639051 WB Image
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody [HL2599] (GTX639051) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX639051 ICC/IF Image
PAX6 antibody [HL2599] detects PAX6 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PAX6 stained by PAX6 antibody [HL2599] (GTX639051) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
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GTX639051 IHC-P Image
PAX6 antibody [HL2599] detects PAX6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: PAX6 stained by PAX6 antibody [HL2599] (GTX639051) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX639051 IHC-P Image
PAX6 antibody [HL2599] detects PAX6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.PAX6 stained by PAX6 antibody [HL2599] (GTX639051) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX639051 IHC-P Image
PAX6 antibody [HL2599] detects PAX6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Green: PAX6 stained by PAX6 antibody [HL2599] (GTX639051) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX639051 IHC-P Image
PAX6 antibody [HL2599] detects PAX6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.PAX6 stained by PAX6 antibody [HL2599] (GTX639051) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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![Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051](/upload/website/prouct_img/small/GTX639051/GTX639051_45292_20240119_WB_D_24012217_256.webp "Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051")
![Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051](/upload/website/prouct_img/small/GTX639051/GTX639051_45292_20240119_WB_TPM_watermark_24012217_553.webp "Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051")
![Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051](/upload/website/prouct_img/small/GTX639051/GTX639051_T-45166_20231117_WB_M_tissue_23112021_216.webp "Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051")
![Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051](/upload/website/prouct_img/small/GTX639051/GTX639051_T-45166_20231117_WB_multiple_B_23112021_171.webp "Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051")
![Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051](/upload/website/prouct_img/small/GTX639051/GTX639051_T-45166_20231117_WB_R_brain_23112021_355.webp "Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051")
![Anti-PAX6 antibody [HL2599] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639051](/upload/website/prouct_img/small/GTX639051/GTX639051_T-45166_20231110_ICC_IF_23121122_635.webp "Anti-PAX6 antibody [HL2599] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639051")
![Anti-PAX6 antibody [HL2599] used in IHC (Paraffin sections) (IHC-P). GTX639051](/upload/website/prouct_img/small/GTX639051/GTX639051_T-45166_20240422_IHC-P_M_1_24043002_128.webp "Anti-PAX6 antibody [HL2599] used in IHC (Paraffin sections) (IHC-P). GTX639051")
![Anti-PAX6 antibody [HL2599] used in IHC (Paraffin sections) (IHC-P). GTX639051](/upload/website/prouct_img/small/GTX639051/GTX639051_T-45166_20240422_IHC-P_M_24043002_919.webp "Anti-PAX6 antibody [HL2599] used in IHC (Paraffin sections) (IHC-P). GTX639051")
![Anti-PAX6 antibody [HL2599] used in IHC (Paraffin sections) (IHC-P). GTX639051](/upload/website/prouct_img/small/GTX639051/GTX639051_T-45166_20240422_IHC-P_R_1_24043002_401.webp "Anti-PAX6 antibody [HL2599] used in IHC (Paraffin sections) (IHC-P). GTX639051")
![Anti-PAX6 antibody [HL2599] used in IHC (Paraffin sections) (IHC-P). GTX639051](/upload/website/prouct_img/small/GTX639051/GTX639051_T-45166_20240422_IHC-P_R_24043002_651.webp "Anti-PAX6 antibody [HL2599] used in IHC (Paraffin sections) (IHC-P). GTX639051")
![Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051](/upload/website/prouct_img/normal/GTX639051/GTX639051_45292_20240119_WB_D_24012217_256.webp "Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051")
![Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051](/upload/website/prouct_img/normal/GTX639051/GTX639051_45292_20240119_WB_TPM_watermark_24012217_553.webp "Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051")
![Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051](/upload/website/prouct_img/normal/GTX639051/GTX639051_T-45166_20231117_WB_M_tissue_23112021_216.webp "Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051")
![Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051](/upload/website/prouct_img/normal/GTX639051/GTX639051_T-45166_20231117_WB_multiple_B_23112021_171.webp "Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051")
![Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051](/upload/website/prouct_img/normal/GTX639051/GTX639051_T-45166_20231117_WB_R_brain_23112021_355.webp "Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051")
![Anti-PAX6 antibody [HL2599] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639051](/upload/website/prouct_img/normal/GTX639051/GTX639051_T-45166_20231110_ICC_IF_23121122_635.webp "Anti-PAX6 antibody [HL2599] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639051")
![Anti-PAX6 antibody [HL2599] used in IHC (Paraffin sections) (IHC-P). GTX639051](/upload/website/prouct_img/normal/GTX639051/GTX639051_T-45166_20240422_IHC-P_M_1_24043002_128.webp "Anti-PAX6 antibody [HL2599] used in IHC (Paraffin sections) (IHC-P). GTX639051")
![Anti-PAX6 antibody [HL2599] used in IHC (Paraffin sections) (IHC-P). GTX639051](/upload/website/prouct_img/normal/GTX639051/GTX639051_T-45166_20240422_IHC-P_M_24043002_919.webp "Anti-PAX6 antibody [HL2599] used in IHC (Paraffin sections) (IHC-P). GTX639051")
![Anti-PAX6 antibody [HL2599] used in IHC (Paraffin sections) (IHC-P). GTX639051](/upload/website/prouct_img/normal/GTX639051/GTX639051_T-45166_20240422_IHC-P_R_1_24043002_401.webp "Anti-PAX6 antibody [HL2599] used in IHC (Paraffin sections) (IHC-P). GTX639051")
![Anti-PAX6 antibody [HL2599] used in IHC (Paraffin sections) (IHC-P). GTX639051](/upload/website/prouct_img/normal/GTX639051/GTX639051_T-45166_20240422_IHC-P_R_24043002_651.webp "Anti-PAX6 antibody [HL2599] used in IHC (Paraffin sections) (IHC-P). GTX639051")
