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PD-L1抗体 [HL1041]

Recombinant antibody KO/KD Comparison Orthogonal Overexpression
Anti-PD-L1 antibody [HL1041] used in IHC (Paraffin sections) (IHC-P). GTX635975
Anti-PD-L1 antibody [HL1041] used in Western Blot (WB). GTX635975
Anti-PD-L1 antibody [HL1041] used in IHC (Paraffin sections) (IHC-P). GTX635975
Anti-PD-L1 antibody [HL1041] used in Western Blot (WB). GTX635975
Anti-PD-L1 antibody [HL1041] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635975
Anti-PD-L1 antibody [HL1041] used in Western Blot (WB). GTX635975
Anti-PD-L1 antibody [HL1041] used in Western Blot (WB). GTX635975
Anti-PD-L1 antibody [HL1041] used in IHC (Paraffin sections) (IHC-P). GTX635975
Anti-PD-L1 antibody [HL1041] used in IHC (Paraffin sections) (IHC-P). GTX635975
Anti-PD-L1 antibody [HL1041] used in IHC (Paraffin sections) (IHC-P). GTX635975
Anti-PD-L1 antibody [HL1041] used in IHC (Paraffin sections) (IHC-P). GTX635975
Anti-PD-L1 antibody [HL1041] used in IHC (Paraffin sections) (IHC-P). GTX635975
Anti-PD-L1 antibody [HL1041] used in IHC (Paraffin sections) (IHC-P). GTX635975

目录号 GTX635975

文献引用 3
1

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1041

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

33 kDa. ( Note )

产品说明

This antibody was raised against human PD-L1 Intracellular domain, and it does not cross with PD-L2 protein.

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Synthetic peptide encompassing a sequence within the Intracellular domain of human PD-L1. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2909927

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

CD274 molecule , B7-H , B7H1 , PD-L1 , PDCD1L1 , PDCD1LG1 , PDL1

细胞定位

Cell membrane

功能与背景

This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

数据库

研究领域

產品图片

Anti-PD-L1 antibody [HL1041] used in IHC (Paraffin sections) (IHC-P). GTX635975

GTX635975 IHC-P Image

PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis.
Sample: Human Placenta.
Green: PD-L1 stained by Dylight594-conjugated PD-L1 antibody [HL1041] (GTX635975) diluted at 1:1000.

Anti-PD-L1 antibody [HL1041] used in Western Blot (WB). GTX635975

GTX635975 WB Image

KO/KD

Wild-type (WT) and PDL1 knockout (KO) MDA-MB-231 cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody [HL1041] (GTX635975) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PD-L1 antibody [HL1041] used in IHC (Paraffin sections) (IHC-P). GTX635975

GTX635975 IHC-P Image

Comparison

PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human placenta.
PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:1000 and competitor's antibody diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

Anti-PD-L1 antibody [HL1041] used in Western Blot (WB). GTX635975

GTX635975 WB Image

Comparison Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody [HL1041] (GTX635975) diluted at 1:2000 and competitor's antibody diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-PD-L1 antibody [HL1041] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635975

GTX635975 ICC/IF Image

PD-L1 antibody [HL1041] detects PD-L1 protein by immunofluorescent analysis.
Sample: MDA-MB-231 cells were fixed in ice-cold MeOH for 10 min.
Green: PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PD-L1 antibody [HL1041] used in Western Blot (WB). GTX635975

GTX635975 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody [HL1041] (GTX635975) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-PD-L1 antibody [HL1041] used in Western Blot (WB). GTX635975

GTX635975 WB Image

Orthogonal Overexpression

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody [HL1041] (GTX635975) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PD-L1 antibody [HL1041] used in IHC (Paraffin sections) (IHC-P). GTX635975

GTX635975 IHC-P Image

PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus (left) and esophageal adenocarcinoma (right).
PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PD-L1 antibody [HL1041] used in IHC (Paraffin sections) (IHC-P). GTX635975

GTX635975 IHC-P Image

PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human lymph node.
PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PD-L1 antibody [HL1041] used in IHC (Paraffin sections) (IHC-P). GTX635975

GTX635975 IHC-P Image

PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human thymus gland.
PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PD-L1 antibody [HL1041] used in IHC (Paraffin sections) (IHC-P). GTX635975

GTX635975 IHC-P Image

PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human placenta.
PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PD-L1 antibody [HL1041] used in IHC (Paraffin sections) (IHC-P). GTX635975

GTX635975 IHC-P Image

PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human ovary.
PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PD-L1 antibody [HL1041] used in IHC (Paraffin sections) (IHC-P). GTX635975

GTX635975 IHC-P Image

PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded A549 (left) and MDA-MB-231 xenograft (right).
PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

Application Reference

HW Chiu et al. J Transl Med 2024; 22 (1) : 13 AIM2 promotes irradiation resistance, migration ability and PD-L1 expression through STAT1/NF-κB activation in oral squamous cell carcinoma
实验应用 : WB
Abdo M et al. Mol Oncol 2023; 17 (5) : 737-746 Comparative evaluation of PD-L1 expression in cytology imprints, circulating tumour cells and tumour tissue in non-small cell lung cancer patients.
Omenai SA et al. PLoS One 2022; 17 (2) : e0263615 Programme death ligand 1 expressions as a surrogate for determining immunotherapy in cervical carcinoma patients.
实验应用 : IHC-P
种属反应 : Human
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PD-L1 antibody [HL1041] 目录号 GTX635975

Rating
( Average 5 based on 1 users reviews)
实验应用
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-PD-L1 antibody [HL1041] used in IHC (Paraffin sections) (IHC-P). GTX635975
Date : Anonymous submitted on 16-Jul-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : Placenta tissue
Primary Antibody : 1:1000, 4°C, 16Hr
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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