产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Bovine, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Full length human PDE6D Recombinant protein
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2910076
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
phosphodiesterase 6D , JBTS22 , PDED
细胞定位
Cytoplasm, cytosol
功能与背景
This gene encodes the delta subunit of rod-specific photoreceptor phosphodiesterase (PDE), a key enzyme in the phototransduction cascade. A similar protein in cow functions in solubilizing membrane-bound PDE. In addition to its role in the PDE complex, the encoded protein is thought to bind to prenyl groups of proteins to target them to subcellular organelles called cilia. Mutations in this gene are associated with Joubert syndrome-22. Alternative splicing results in multiple splice variants. [provided by RefSeq, Mar 2014]
数据库
研究领域
產品图片
![Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799](/templates/default/js/lightbox/images/loading.gif "Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799")
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GTX636799 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [HL1359] (GTX636799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636799 IHC-P Image
PDE6D antibody [HL1359] detects PDE6D protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded cat colon.PDE6D stained by PDE6D antibody [HL1359] (GTX636799) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636799 ICC/IF Image
PDE6D antibody [HL1359] detects PDE6D protein at nucleus by immunofluorescent analysis.Sample: MOLT-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PDE6D stained by PDE6D antibody [HL1359] (GTX636799) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636799 WB Image
Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [HL1359] (GTX636799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636799 IHC-P Image
PDE6D antibody [HL1359] detects PDE6D protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: PDE6D stained by PDE6D antibody [HL1359] (GTX636799) diluted at 1:100.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636799 WB Image
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [HL1359] (GTX636799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX636799 WB Image
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [HL1359] (GTX636799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
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GTX636799 IHC-P Image
PDE6D antibody [HL1359] detects PDE6D protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
PDE6D stained by PDE6D antibody [HL1359] (GTX636799) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
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GTX636799 IHC-P Image
PDE6D antibody [HL1359] detects PDE6D protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
PDE6D stained by PDE6D antibody [HL1359] (GTX636799) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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|
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GTX636799 WB Image
Whole zebrafish extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [HL1359] (GTX636799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799](/upload/website/prouct_img/small/GTX636799/GTX636799_44648_20221223_WB_D_C_22122722_604.webp "Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799")
![Anti-PDE6D antibody [HL1359] used in IHC (Paraffin sections) (IHC-P). GTX636799](/upload/website/prouct_img/small/GTX636799/GTX636799_44648_20230331_IHC-P_Cat_23032819_237.webp "Anti-PDE6D antibody [HL1359] used in IHC (Paraffin sections) (IHC-P). GTX636799")
![Anti-PDE6D antibody [HL1359] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636799](/upload/website/prouct_img/small/GTX636799/GTX636799_44648_20230512_ICC_IF_23060622_954.webp "Anti-PDE6D antibody [HL1359] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636799")
![Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799](/upload/website/prouct_img/small/GTX636799/GTX636799_T-44599_20220311_WB_M_eye_w_23061202_140.webp "Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799")
![Anti-PDE6D antibody [HL1359] used in IHC (Paraffin sections) (IHC-P). GTX636799](/upload/website/prouct_img/small/GTX636799/GTX636799_T-44599_20220415_IHC-P_M_w_23061202_615.webp "Anti-PDE6D antibody [HL1359] used in IHC (Paraffin sections) (IHC-P). GTX636799")
![Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799](/upload/website/prouct_img/small/GTX636799/GTX636799_44648_20220422_WB_w_23061202_500.webp "Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799")
![Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799](/upload/website/prouct_img/small/GTX636799/GTX636799_44648_20220506_WB_w_23061202_156.webp "Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799")
![Anti-PDE6D antibody [HL1359] used in IHC (Paraffin sections) (IHC-P). GTX636799](/upload/website/prouct_img/small/GTX636799/GTX636799_44648_20220520_IHC-P_M_w_23061202_895.webp "Anti-PDE6D antibody [HL1359] used in IHC (Paraffin sections) (IHC-P). GTX636799")
![Anti-PDE6D antibody [HL1359] used in IHC (Paraffin sections) (IHC-P). GTX636799](/upload/website/prouct_img/small/GTX636799/GTX636799_44648_20220520_IHC-P_R_w_23061202_224.webp "Anti-PDE6D antibody [HL1359] used in IHC (Paraffin sections) (IHC-P). GTX636799")
![Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799](/upload/website/prouct_img/small/GTX636799/GTX636799_44648_20221216_WB_Z_22122018_543.webp "Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799")
![Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799](/upload/website/prouct_img/normal/GTX636799/GTX636799_44648_20221223_WB_D_C_22122722_604.webp "Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799")
![Anti-PDE6D antibody [HL1359] used in IHC (Paraffin sections) (IHC-P). GTX636799](/upload/website/prouct_img/normal/GTX636799/GTX636799_44648_20230331_IHC-P_Cat_23032819_237.webp "Anti-PDE6D antibody [HL1359] used in IHC (Paraffin sections) (IHC-P). GTX636799")
![Anti-PDE6D antibody [HL1359] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636799](/upload/website/prouct_img/normal/GTX636799/GTX636799_44648_20230512_ICC_IF_23060622_954.webp "Anti-PDE6D antibody [HL1359] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636799")
![Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799](/upload/website/prouct_img/normal/GTX636799/GTX636799_T-44599_20220311_WB_M_eye_w_23061202_140.webp "Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799")
![Anti-PDE6D antibody [HL1359] used in IHC (Paraffin sections) (IHC-P). GTX636799](/upload/website/prouct_img/normal/GTX636799/GTX636799_T-44599_20220415_IHC-P_M_w_23061202_615.webp "Anti-PDE6D antibody [HL1359] used in IHC (Paraffin sections) (IHC-P). GTX636799")
![Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799](/upload/website/prouct_img/normal/GTX636799/GTX636799_44648_20220422_WB_w_23061202_500.webp "Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799")
![Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799](/upload/website/prouct_img/normal/GTX636799/GTX636799_44648_20220506_WB_w_23061202_156.webp "Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799")
![Anti-PDE6D antibody [HL1359] used in IHC (Paraffin sections) (IHC-P). GTX636799](/upload/website/prouct_img/normal/GTX636799/GTX636799_44648_20220520_IHC-P_M_w_23061202_895.webp "Anti-PDE6D antibody [HL1359] used in IHC (Paraffin sections) (IHC-P). GTX636799")
![Anti-PDE6D antibody [HL1359] used in IHC (Paraffin sections) (IHC-P). GTX636799](/upload/website/prouct_img/normal/GTX636799/GTX636799_44648_20220520_IHC-P_R_w_23061202_224.webp "Anti-PDE6D antibody [HL1359] used in IHC (Paraffin sections) (IHC-P). GTX636799")
![Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799](/upload/website/prouct_img/normal/GTX636799/GTX636799_44648_20221216_WB_Z_22122018_543.webp "Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799")
