产品说明
摘要
PSD95 antibody recognizes postsynaptic density protein 95, an ~80 kDa multidomain scaffold protein enriched at neuron postsynaptic excitatory membranes. It belongs to the membrane-associated guanylate kinase (MAGUK) family, which also contains PSD-93, SAP97 and SAP102. PSD95 is one of the most abundant proteins in the PSD, and functions in synapse maturation, strength, and plasticity.
产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
mouse brain , mouse fetal brain , rat brain , mouse eye
预测种属反应
Bovine(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.39 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Mouse
免疫原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse PSD95. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2886821
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
discs large MAGUK scaffold protein 4 , Dlgh4 , PSD-95 , PSD95 , SAP90 , SAP90A
细胞定位
Membrane , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density , synapse
功能与背景
Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ACCN3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
数据库
研究领域
產品图片
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GTX133091 IHC-Fr Image
PSD95 antibody detects PSD95 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: PSD95 protein stained by PSD95 antibody (GTX133091) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX133091 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PSD95 antibody (GTX133091) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX133091 WB Image
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PSD95 antibody (GTX133091) diluted at 1:1000.
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GTX133091 IHC-P Image
PSD95 antibody detects PSD95 protein expression by immunohistochemical analysis.
Sample: Paraffin-Embedded adult mouse retina.
Green: PSD95 protein stained by PSD95 antibody (GTX133091) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX133091 ICC/IF Image
PSD95 antibody detects PSD95 protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PSD95 protein stained by PSD95 antibody (GTX133091) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX133091 IHC-P Image
PSD95 antibody detects PSD95 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: PSD95 stained by PSD95 antibody (GTX133091) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1 , a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX133091 WB Image
The data was published in the 2022 in Front Aging Neurosci. PMID: 35264943
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文献引用
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