产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
HeLa , A431 , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2
预测种属反应
Chicken, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide corresponding to residues around human Paxillin (non-phospho Tyr118). The exact sequence is proprietary.
纯化方式
Affinity purified by Protein G.
偶联
Unconjugated
RRID
AB_2888372
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
paxillin
细胞定位
Cytoplasm , cytoskeleton , Cell junction , focal adhesion
数据库
研究领域
產品图片
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GTX633707 WB Image
Wild-type (WT) and Cyclin D1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
The observed effect is based on the publication: PMID: 16705174
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GTX633707 ICC/IF Image
Paxillin (non-phospho Tyr118) antibody [GT722] detects Paxillin (non-phospho Tyr118) protein at focal adhesion by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Paxillin (non-phospho Tyr118) stained by Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Blue: Hoechst 33342 staining.
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GTX633707 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX633707 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:1000.
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GTX633707 WB Image
Untreated (–) and treated (+) A431 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX633707 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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