Pcna抗体

Name: Pcna抗体
Brand: GeneTex
SKU: GTX124496
Price: 4000.00 RMB
Rating: 4 (1 reviews)

Anti-Pcna antibody used in Western Blot (WB). GTX124496

Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496

Anti-Pcna antibody used in IHC (Paraffin sections) (IHC-P). GTX124496

目录号 GTX124496

文献引用 21

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, IHC-P, IHC-Fr, IHC-Wm
种属反应	Zebrafish, Japanese Medaka, Nothobranchius furzeri
产品包装	100 μl (¥4000), 25 μl (¥1700)

查看所有 zebrafish Pcna 的产品

View product citations for antibody GTX124496 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:3000
IHC-P	1:100-1:1000
IHC-Fr	Assay dependent
IHC-Wm	1:50-1:500

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

29 kDa. ( Note )

阳性对照

zebrafish heart , zebrafish muscle , whole zebrafish , zebrafish muscle

预测种属反应

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原

The immunogen used to generate this antibody corresponds to zebrafish Pcna

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11161916

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

proliferating cell nuclear antigen , cb16 , fa28e03 , fb36g03 , wu:fa28e03 , wu:fb36g03

细胞定位

Nucleus

功能与背景

This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.

数据库

Gene ID: 30678 pcna
UniProt: Q9PTP1 pcna

研究领域

- 斑马鱼科研

產品图片

GTX124496 WB Image

Various tissue extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Pcna antibody (GTX124496) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124496 IHC-Wm Image

Pcna antibody detects Pcna protein on zebrafish by whole mount immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
Pcna antibody (GTX124496) dilution: 1:50.

GTX124496 IHC-Wm Image

Pcna antibody detects Pcna protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 2 day-post-fertilization zebrafish embryo.
Pcna antibody (GTX124496) dilution: 1:100.

GTX124496 IHC-Wm Image

Pcna antibody detects Pcna protein on Medaka by whole mount immunohistochemical analysis.
Sample: 7 days-post-fertilization medaka embryo.
Pcna antibody (GTX124496) dilution: 1:100.

GTX124496 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish kidney, using pcna (GTX124496) antibody at 1:300 dilution.

GTX124496 WB Image

The data was published in the journal Cells in 2019.PMID: 31569376

GTX124496 IHC-Wm Image

The data was published in the journal Development in 2018.PMID: 29695612

GTX124496 IHC-Wm Image

The data was published in the journal Development in 2018.PMID: 29695612

GTX124496 IHC-Wm Image

The data was published in the journal Development in 2018.PMID: 29695612

GTX124496 IHC-Wm Image

The data was published in the 2019 in Front Mol Neurosci. PMID: 31616250

文献引用

Application Reference

Saraswathy VM et al. Cell Rep 2022; 41 (8) : 111705 Myostatin is a negative regulator of adult neurogenesis after spinal cord injury in zebrafish.

Ellis PS et al. Immun Ageing 2022; 19 (1) : 31 A subset of gut leukocytes has telomerase-dependent "hyper-long" telomeres and require telomerase for function in zebrafish.

Raquel R Martins et al. Aging Cell 2022; 21 (4) : e13597 M?ller Glia maintain their regenerative potential despite degeneration in the aged zebrafish retina.

实验应用 : IHC-Fr

种属反应 : Zebrafish

Nicolas Dray et al. Cell Stem Cell 2021; 28 (8) : 1457-1472.e12 Dynamic spatiotemporal coordination of neural stem cell fate decisions occurs through local feedback in the adult vertebrate brain.

实验应用 : IHC-Wm

种属反应 : Zebrafish

Klatt Shaw D et al. Dev Cell 2021; 56 (5) : 613-626.e7 Localized EMT reprograms glial progenitors to promote spinal cord repair.

实验应用 : IHC-Fr

种属反应 : Zebrafish

显示更多 +

我要回报文献

产品评论

Pcna antibody 目录号 GTX124496

Rating

( Average 4 based on 1 users reviews)

实验应用

IHC (Frozen sections)(IHC-Fr)

( Average 4 based on 1 users reviews)

Date : Anonymous submitted on 03-Aug-2018

Score :

Application Tested : IHC-Fr

Sample Species : Zfsh

Sample : 3 days post amputation(dpa) zebrafish caudal fin blastema

Blocking : 1.5% skim milk, 25°C, 0.5Hr

Fixation : 4% PFA/ PBS

Permeabilization /Antigen retrieval : 10 mM Citrate buffer, 105°C, 1 minHr

Primary Antibody : 1:500, 4°C, 16Hr

Figure Note : Red: Pcna stained by Pcna antibody (GTX124496) diluted at 1:500. Blue: DAPI

我要评论产品

Application Reference

Saraswathy VM et al. Cell Rep 2022; 41 (8):111705 Myostatin is a negative regulator of adult neurogenesis after spinal cord injury in zebrafish.

Ellis PS et al. Immun Ageing 2022; 19 (1):31 A subset of gut leukocytes has telomerase-dependent "hyper-long" telomeres and require telomerase for function in zebrafish.

Raquel R Martins et al. Aging Cell 2022; 21 (4):e13597 M?ller Glia maintain their regenerative potential despite degeneration in the aged zebrafish retina.

实验应用 : IHC-Fr

种属反应 : Zebrafish

Nicolas Dray et al. Cell Stem Cell 2021; 28 (8):1457-1472.e12 Dynamic spatiotemporal coordination of neural stem cell fate decisions occurs through local feedback in the adult vertebrate brain.

实验应用 : IHC-Wm

种属反应 : Zebrafish

Klatt Shaw D et al. Dev Cell 2021; 56 (5):613-626.e7 Localized EMT reprograms glial progenitors to promote spinal cord repair.

实验应用 : IHC-Fr

种属反应 : Zebrafish

Hromowyk KJ et al. Dev Biol 2020; 462 (1):85-100 Cell fusion is differentially regulated in zebrafish post-embryonic slow and fast muscle.

Coolen M et al. Curr Biol 2020; 30 (4):736-745.e4 Mosaic Heterochrony in Neural Progenitors Sustains Accelerated Brain Growth and Neurogenesis in the Juvenile Killifish N.?furzeri.

Oyarbide U et al. JCI Insight 2020; 5 (17) Loss of Sbds in zebrafish leads to neutropenia and pancreas and liver atrophy.

实验应用 : IHC-P

种属反应 : Zebrafish

Adriana Petrovici et al. J. Mar. Sci. Eng. 2020; 8 (2):73 Toxicity of Deltamethrin to Zebrafish Gonads Revealed by Cellular Biomarkers.

实验应用 : IHC-P

种属反应 : Zebrafish

K?nig D et al. Front Mol Neurosci 2019; 12:227 Distribution and Restoration of Serotonin-Immunoreactive Paraneuronal Cells During Caudal Fin Regeneration in Zebrafish.

实验应用 : IHC-Wm

Cencioni C et al. Cells 2019; 8 (10) Aging Triggers H3K27 Trimethylation Hoarding in the Chromatin of Nothobranchius furzeri Skeletal Muscle.

实验应用 : WB

Zhang Z et al. Glia 2019; (Epub) Inflammation-induced mammalian target of rapamycin signaling is essential for retina regeneration.

实验应用 : IHC-Fr

种属反应 : Zebrafish

Cencioni, C. et al. Preprints 2019;:2019080032 Aging Triggers H3K27 Trimethylation Hoarding in the Chromatin of Nothobranchius Furzeri Skeletal Muscle.

实验应用 : WB

D?sir?e K?nig et al. bioRxiv 2019; (Epub) Distribution and restoration of serotonin-immunoreactive paraneuronal cells during caudal fin regeneration in zebrafish.

实验应用 : IHC-Wm

种属反应 : Zebrafish

Xiao D et al. PLoS Genet 2018; 14 (8):e1007578 The roles of SMYD4 in epigenetic regulation of cardiac development in zebrafish.

实验应用 : IHC-Wm

种属反应 : Zebrafish

Than-Trong E et al. Development 2018; 145 (10) Neural stem cell quiescence and stemness are molecularly distinct outputs of the Notch3 signalling cascade in the vertebrate adult brain.

实验应用 : IHC-Wm

种属反应 : Zebrafish

Furlan G et al. Curr Biol 2017; 27 (21):3288-3301.e3 Life-Long Neurogenic Activity of Individual Neural Stem Cells and Continuous Growth Establish an Outside-In Architecture in the Teleost Pallium.

实验应用 : IHC-Wm

种属反应 : Zebrafish

Katz S et al. Cell Rep 2016; 17 (5):1383-1398 A Nuclear Role for miR-9 and Argonaute Proteins in Balancing Quiescent and Activated Neural Stem Cell States.

种属反应 : Zebrafish

Zhang S et al. Invest Ophthalmol Vis Sci 2016; 57 (4):1991-2000 Antiviral Drug * Is a Potent Inhibitor of the Proliferation of Mller Glia-Derived Progenitors During Zebrafish Retinal Regeneration.

实验应用 : WB

种属反应 : Zebrafish

Dray N et al. Development 2015; 142 (20):3592-600 Large-scale live imaging of adult neural stem cells in their endogenous niche.

种属反应 : Zebrafish

Panza P et al. Development 2015; 142 (10):1879-84 Live imaging of endogenous protein dynamics in zebrafish using chromobodies.

实验应用 : IHC-Wm

种属反应 : Zebrafish

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

	(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
	http://www.neobioscience.com
	genetex@neobioscience.com

Pcna抗体

目录号 GTX124496

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX124496 WB Image

GTX124496 IHC-Wm Image

GTX124496 IHC-Wm Image

GTX124496 IHC-Wm Image

GTX124496 IHC-Wm Image

GTX124496 IHC-P Image

GTX124496 WB Image

GTX124496 IHC-Wm Image

GTX124496 IHC-Wm Image

GTX124496 IHC-Wm Image

GTX124496 IHC-Wm Image

文献引用

Application Reference

产品评论

Pcna antibody 目录号 GTX124496

产品

研究

支持

公司

Follow Us

粤ICP备08007346号-9

© 1998-2022 GeneTex, Inc. 保留所有权利。

所有产品仅供研究使用 - 不应用于诊断或治疗。