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RAB11B抗体 [N1C3]

Anti-RAB11B antibody [N1C3] used in Western Blot (WB). GTX119095
Anti-RAB11B antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119095
Anti-RAB11B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119095
Anti-RAB11B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119095
Anti-RAB11B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119095
Anti-RAB11B antibody [N1C3] used in IHC (Frozen sections) (IHC-Fr). GTX119095
Anti-RAB11B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119095
Anti-RAB11B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119095
Anti-RAB11B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119095
Anti-RAB11B antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119095
Anti-RAB11B antibody [N1C3] used in Western Blot (WB). GTX119095
Anti-RAB11B antibody [N1C3] used in Western Blot (WB). GTX119095

目录号 GTX119095

1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 RAB11B 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

24 kDa. ( Note )

预测种属反应

Drosophila, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.67 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human RAB11B. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10619861

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

RAB11B, member RAS oncogene family , H-YPT3 , NDAGSCW

细胞定位

Cell membrane; Lipid-anchor; Cytoplasmic side

功能与背景

The Ras superfamily of small GTP-binding proteins, which includes the Ras (see MIM 190020), Ral (see MIM 179550), Rho (see MIM 165390), Rap (see MIM 179520), and Rab (see MIM 179508) families, is involved in controlling a diverse set of essential cellular functions. The Rab family, including RAB11B, appears to play a critical role in regulating exocytotic and endocytotic pathways (summary by Zhu et al., 1994 [PubMed 7811277]).[supplied by OMIM]

数据库

研究领域

產品图片

Anti-RAB11B antibody [N1C3] used in Western Blot (WB). GTX119095

GTX119095 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody [N1C3] (GTX119095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAB11B antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119095

GTX119095 ICC/IF Image

RAB11B antibody [N1C3] detects RAB11B protein by immunofluorescent analysis.
Sample: DIV9 rat cortical neuron and Glia cell cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAB11B stained by RAB11B antibody [N1C3] (GTX119095) diluted at 1:250.
Red: Tau, a Axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-RAB11B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119095

GTX119095 IHC-P Image

RAB11B antibody [N1C3] detects RAB11B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
RAB11B stained by RAB11B antibody [N1C3] (GTX119095) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB11B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119095

GTX119095 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RAB11B antibody(GTX119095) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RAB11B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119095

GTX119095 IHC-P Image

RAB11B antibody [N1C3] detects RAB11B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
RAB11B stained by RAB11B antibody [N1C3] (GTX119095) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB11B antibody [N1C3] used in IHC (Frozen sections) (IHC-Fr). GTX119095

GTX119095 IHC-Fr Image

RAB11B antibody [N1C3] detects RAB11B Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: RAB11B stained by RAB11B antibody [N1C3] (GTX119095) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-RAB11B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119095

GTX119095 IHC-P Image

RAB11B antibody [N1C3] detects RAB11B protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
RAB11B antibody [N1C3] (GTX119095) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB11B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119095

GTX119095 IHC-P Image

RAB11B antibody [N1C3] detects RAB11B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
RAB11B stained by RAB11B antibody [N1C3] (GTX119095) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB11B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119095

GTX119095 IHC-P Image

RAB11B antibody [N1C3] detects RAB11B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
RAB11B stained by RAB11B antibody [N1C3] (GTX119095) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB11B antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119095

GTX119095 ICC/IF Image

GRAMD1B antibody [C1C2], Internal detects GRAMD1B protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde at RT for 15 min.
Green: GRAMD1B protein stained by GRAMD1B antibody [C1C2], Internal (GTX119095) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-RAB11B antibody [N1C3] used in Western Blot (WB). GTX119095

GTX119095 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody [N1C3] (GTX119095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAB11B antibody [N1C3] used in Western Blot (WB). GTX119095

GTX119095 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody [N1C3] (GTX119095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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RAB11B antibody [N1C3] 目录号 GTX119095

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实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-RAB11B antibody [N1C3] used in Western Blot (WB). GTX119095
Date : Anonymous submitted on 09-May-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 10 μg
Blocking : 5% skim milk in TBST, 27°C, 1Hr
Primary Antibody : 1:5000, 4°C, 12Hr
Secondary Antibody : GTX213110-01, 1:5000, 27°C, 2Hr
条带说明 : Two groups (compound A -/+) of whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody (GTX119095) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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