产品说明
摘要
S100B, a member of the S100 family of calcium-binding cytosolic proteins with a predicted molecular weight of 11kDa, is an independent prognostic marker for melanoma. Elevated S100B and lactate dehydrogenase (LDH) levels are clinically useful for the detection of tumor progression and metastasis.
产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
V5-human S100B-transfected 293T cells
预测种属反应
Rabbit, Bovine, Chicken, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.14 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Full length human S100 beta Recombinant protein.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2886037
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
S100 calcium binding protein B , NEF , S100 , S100-B , S100beta
细胞定位
Cytoplasm , Nucleus
功能与背景
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX129573 IHC-P Image
S100 beta antibody detects S100 beta protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.S100 beta stained by S100 beta antibody (GTX129573) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX129573 WB Image
S100B antibody detects S100B protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of V5-human S100B-transfected 293T cells
15 % SDS-PAGE
S100B antibody (GTX129573) dilution: 1:1000
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GTX129573 ICC/IF Image
S100 beta antibody detects S100 beta protein at glia cells by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: S100 beta stained by S100 beta antibody (GTX129573) diluted at 1:500.Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX129573 IHC-P Image
S100 beta antibody detects S100 beta protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human skin.S100 beta stained by S100 beta antibody (GTX129573) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX129573 IHC-Fr Image
S100 beta antibody detects S100 beta protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: S100 beta stained by S100 beta antibody (GTX129573) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX129573 IHC-P Image
S100B antibody detects S100B protein at glial cell on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
S100B antibody (GTX129573) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX129573 WB Image
Mouse tissue extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with S100 beta antibody (GTX129573) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX129573 IHC-P Image
S100 beta antibody detects S100 beta protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.S100 beta stained by S100 beta antibody (GTX129573) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX129573 WB Image
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with S100 beta antibody (GTX129573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX129573 WB Image
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with S100 beta antibody (GTX129573) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX129573 WB Image
The data was published in the journal PeerJ in 2018. PMID: 29888131
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文献引用
产品评论
S100 beta antibody
目录号 GTX129573
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Rating
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( Average 4.5 based on 2 users reviews)
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实验应用
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IHC (Frozen sections)(IHC-Fr)
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( Average 4.5 based on 2 users reviews)
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Date :
Anonymous submitted on 22-Sep-2023
Application Tested :
IHC-Fr
Sample Species :
Rat
Sample :
rat sciatic nerve
Blocking :
5% goat serum in PBS, 37°C, 0.5Hr
Fixation :
4% PFA, 25°C, 16Hr
Primary Antibody :
1:500, 37°C, 1.5Hr
Date :
Anonymous submitted on 09-Nov-2021
Application Tested :
IHC-Fr
Sample Species :
Ms
Sample :
Mouse small intestine tissue
Fixation :
4% PFA in PBS
Permeabilization /Antigen retrieval :
Blocking One Histo (Nacalai Tesque), 10min, 25°C
Primary Antibody :
1:250, 4°C, 16Hr
