产品说明
摘要
3,3′,5,5′-Tetramethylbenzidine (TMB) is a chromogenic substrate suitable for use in ELISA procedures, which utilize horseradish peroxidase conjugates. This substrate produces a soluble end product that is blue in color and can be read spectrophotometrically at 370 or 655 nm. The reaction maybe stopped with acid, resulting in a yellow solution that is read at 450 nm. This product is supplied as a ready-to-use peroxidase substrate containing TMB in a mildly acidic buffer. Prior to reaction with peroxidase, the substrate should be a colorless to light bluish-green solution. The substrate system develops a blue reaction product when reacted with peroxidase in microwell applications (e.g. ELISA assays). For end-point assays, acid can be used to stop the reaction, to yield a yellow end product. Since this substrate produces a soluble reaction product, it is not recommended for histochemistry or blotting.
产品应用
应用说明
Following reaction with peroxidase, a blue reaction product forms that may be read at 370 nm or between 620 and 655 nm. For end-point assays, the reaction can be stopped by the addition of a volume of 1 M or 2 M HCl, or 0.5 M H2SO4, equal to the volume of the substrate reaction in the well. The resulting yellow end product, which is stable for at least one hour, can then be read at 450 nm. Dilution of the substrate is not recommended. To reduce the intensity of a reaction, it is recommended that the antibodies or conjugates be diluted.
产品属性
形式
Liquid
存储溶液
TMB is a combination of buffer, chromogen and H₂O₂, as a substrate, in one convenient single component reagent.
保存剂
No preservative
存放说明
Store at 4ºC. Protect from light.
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
功能与背景
3-3',5,5'-tetramethylbenzidine (TMB) is a soluble chromogen substrate for horseradish peroxidase detection systems. It is recommended for ELISA procedures.
產品图片
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GTX78422 Image
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文献引用
产品评论
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