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TOMM20抗体

Comparison Orthogonal
Anti-TOMM20 antibody used in Western Blot (WB). GTX133756
Anti-TOMM20 antibody used in Western Blot (WB). GTX133756
Anti-TOMM20 antibody used in Western Blot (WB). GTX133756
Anti-TOMM20 antibody used in Western Blot (WB). GTX133756
Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756
Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756
Anti-TOMM20 antibody used in Western Blot (WB). GTX133756
Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756
Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756
Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756
Anti-TOMM20 antibody used in Western Blot (WB). GTX133756
Anti-TOMM20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133756
Anti-TOMM20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133756
Anti-TOMM20 antibody used in Western Blot (WB). GTX133756
Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756

目录号 GTX133756

文献引用 3
4

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

16 kDa. ( Note )

阳性对照

293T , A431 , HeLa , HepG2 , mouse kidney , rat thymus

预测种属反应

Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Full length Human TOMM20 recombinant protein.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2887094

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

translocase of outer mitochondrial membrane 20 , MAS20 , MOM19 , TOM20

细胞定位

Mitochondrion outer membrane; Single-pass membrane protein

功能与背景

Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.

数据库

研究领域

產品图片

Anti-TOMM20 antibody used in Western Blot (WB). GTX133756

GTX133756 WB Image

Comparison

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membranes were blotted with TOMM20 antibody (GTX133756) diluted at 1:1000 and competitor's antibody (Competitor A) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-TOMM20 antibody used in Western Blot (WB). GTX133756

GTX133756 WB Image

Orthogonal

HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with TOMM20 antibody (GTX133756) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TOMM20 antibody used in Western Blot (WB). GTX133756

GTX133756 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with TOMM20 antibody (GTX133756) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TOMM20 antibody used in Western Blot (WB). GTX133756

GTX133756 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with TOMM20 antibody (GTX133756) diluted at 1:1000 and competitor's antibody (sc-11415) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756

GTX133756 IHC-P Image

TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
TOMM20 stained by TOMM20 antibody (GTX133756) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756

GTX133756 IHC-P Image

TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
TOMM20 stained by TOMM20 antibody (GTX133756) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TOMM20 antibody used in Western Blot (WB). GTX133756

GTX133756 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with TOMM20 antibody (GTX133756) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756

GTX133756 IHC-P Image

TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
TOMM20 stained by TOMM20 antibody (GTX133756) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756

GTX133756 IHC-P Image

TOMM20 antibody detects TOMM20 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
TOMM20 stained by TOMM20 antibody (GTX133756) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756

GTX133756 IHC-P Image

TOMM20 antibody detects TOMM20 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
TOMM20 stained by TOMM20 antibody (GTX133756) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TOMM20 antibody used in Western Blot (WB). GTX133756

GTX133756 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with TOMM20 antibody (GTX133756) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TOMM20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133756

GTX133756 ICC/IF Image

TOMM20 antibody detects TOMM20 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TOMM20 stained by TOMM20 antibody (GTX133756) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-TOMM20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133756

GTX133756 ICC/IF Image

TOMM20 antibody detects TOMM20 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TOMM20 stained by TOMM20 antibody (GTX133756) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-TOMM20 antibody used in Western Blot (WB). GTX133756

GTX133756 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with TOMM20 antibody (GTX133756) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756

GTX133756 IHC-P Image

TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
TOMM20 stained by TOMM20 antibody (GTX133756) diluted at 1:615.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

Application Reference

Chen KC et al. J Clin Med 2021; 11 (1) Bicalutamide Exhibits Potential to Damage Kidney via Destroying Complex I and Affecting Mitochondrial Dynamics.
实验应用 : WB
种属反应 : Rat
Bosc C et al. Nat Commun 2020; 11 (1) : 4056 Autophagy regulates fatty acid availability for oxidative phosphorylation through mitochondria-endoplasmic reticulum contact sites.
实验应用 : ICC/IF
种属反应 : Human
Keller G et al. Neurobiol Dis 2018; 124 : 57-66 Mitochondrial dysfunction in preclinical genetic prion disease: A target for preventive treatment?
实验应用 : IHC-P
种属反应 : Mouse
我要回报文献

产品评论

TOMM20 antibody 目录号 GTX133756

Rating
( Average 4.8 based on 4 users reviews)
实验应用
Western Blot(WB)
( Average 4.7 based on 3 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-TOMM20 antibody used in Western Blot (WB). GTX133756
Date : Anonymous submitted on 12-Nov-2023
Score :
Application Tested : WB
Sample Species : Ms
Sample : Mouse Brain tissue
Amount used : 10μg
Blocking : 5% non-fat milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:500, 25°C, 16Hr
Anti-TOMM20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133756
Date : Anonymous submitted on 03-May-2023
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HCT116 cells
Blocking : PBS, 2% BSA, 2% FBS, 0.1% Tween20, 0.02% NaN3, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.2% Triton X-100
Primary Antibody : 1:200, 25°C, 1Hr
Anti-TOMM20 antibody used in Western Blot (WB). GTX133756
Date : Anonymous submitted on 14-Oct-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U937 cells
Amount used : 80μg
Blocking : PVDF Blocking reagent, RT, 1Hr
Primary Antibody : 1:3000, 4°C, > 12Hr
Anti-TOMM20 antibody used in Western Blot (WB). GTX133756
Date : Anonymous submitted on 12-May-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : Synapotosome isolated from mouse brain tissue
Amount used : 10-20 μg
Blocking : 3% milk powder in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 8Hr
Figure Note : Numbers indicated separate mice; L: left and R: right hippocampus and prefrontal cortex (PFC).
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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