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Tau (phospho Thr217)抗体 [HL1276]

Recombinant antibody Orthogonal
Anti-Tau (phospho Thr217) antibody [HL1276] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636678
Anti-Tau (phospho Thr217) antibody [HL1276] used in Western Blot (WB). GTX636678
Anti-Tau (phospho Thr217) antibody [HL1276] used in (). GTX636678
Anti-Tau (phospho Thr217) antibody [HL1276] used in IHC (Paraffin sections) (IHC-P). GTX636678
Anti-Tau (phospho Thr217) antibody [HL1276] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636678

目录号 GTX636678

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1276

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Tau 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

79 kDa. ( Note )

预测种属反应

Bovine, Dog, Chicken, Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide surrounding phospho Thr217 of human Tau. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2910044

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

microtubule associated protein tau , DDPAC , FTDP-17 , MAPTL , MSTD , MTBT1 , MTBT2 , PPND , PPP1R103 , TAU

细胞定位

Cytoplasm, cytosol,Cell membrane,Cytoplasm, cytoskeleton,Cytoplasm,Cell projection, dendrite

功能与背景

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-Tau (phospho Thr217) antibody [HL1276] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636678

GTX636678 ICC/IF Image

Tau (phospho Thr217) antibody [HL1276] detects Tau (phospho Thr217) protein at cytoskeleton by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tau (phospho Thr217) stained by Tau (phospho Thr217) antibody [HL1276] (GTX636678) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Tau (phospho Thr217) antibody [HL1276] used in Western Blot (WB). GTX636678

GTX636678 WB Image

Orthogonal

Untreated (–) and treated (+) SK-N-SH whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr217) antibody [HL1276] (GTX636678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Tau (phospho Thr217) antibody [HL1276] used in (). GTX636678

GTX636678 Image

Indirect ELISA analysis was performed by coating the plate with Tau peptide and Tau (phospho Thr217) peptide (1000-15.63 ng/mL). Coated peptides were probed with Tau (phospho Thr217) antibody [HL1276] (GTX636678) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Anti-Tau (phospho Thr217) antibody [HL1276] used in IHC (Paraffin sections) (IHC-P). GTX636678

GTX636678 IHC-P Image

Tau (phospho Thr217) antibody [HL1276] detects Tau (phospho Thr217) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Tau (phospho Thr217) stained by Tau (phospho Thr217) antibody [HL1276] (GTX636678) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Tau (phospho Thr217) antibody [HL1276] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636678

GTX636678 ICC/IF Image

Tau (phospho Thr217) antibody [HL1276] detects Tau (phospho Thr217) protein at cytoskeleton by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tau (phospho Thr217) stained by Tau (phospho Thr217) antibody [HL1276] (GTX636678) diluted at 1:250.
Red: Tau, a cytoskeleton marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

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产品包装 报价 (¥)
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¥ 1700
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