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Ubiquitin抗体 [GT7811]

Comparison Orthogonal Overexpression
Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148
Anti-Ubiquitin antibody [GT7811] used in IHC (Paraffin sections) (IHC-P). GTX630148
Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148
Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148
Anti-Ubiquitin antibody [GT7811] used in IHC (Paraffin sections) (IHC-P). GTX630148
Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148
Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148
Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148

目录号 GTX630148

文献引用 2

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT7811

同种型

 IgG2b

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:5000-1:20000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

阳性对照

Myc-human Ubiquition-transfected 293T cells , 293T , A431 , HepG2 , HeLa (10 μM MG132 treatment for 16 hr) , Neuro2A , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2

预测种属反应

Yeast, Drosophila, Bovine, Chicken(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原

The immunogen used to generate this antibody corresponds to Ubiquitin.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888189

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

研究领域

產品图片

Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148

GTX630148 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:5000.

Anti-Ubiquitin antibody [GT7811] used in IHC (Paraffin sections) (IHC-P). GTX630148

GTX630148 IHC-P Image

Ubiquitin antibody [GT7811] detects Ubiquitin protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Ubiquitin stained by Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148

GTX630148 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148

GTX630148 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Ubiquitin antibody [GT7811] used in IHC (Paraffin sections) (IHC-P). GTX630148

GTX630148 IHC-P Image

Ubiquitin antibody [GT7811] detects Ubiquitin protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Ubiquitin stained by Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148

GTX630148 WB Image

Overexpression

Ubiquitin antibody detects Ubiquitin protein by western blot analysis. Non-transfected (-) and -transfected (+, including Myc-tag) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Ubiquitin antibody (GTX630148) diluted by 1:10000.

Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148

GTX630148 WB Image

Orthogonal

293T whole cell extracts and 293T exosome extract (2.6 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:250. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148

GTX630148 WB Image

Comparison Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:500 and competitor's antibody (sc-53509) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

文献引用

Application Reference

Mhoriam Ahmed et al. Research Square 2023; Amplifying the heat shock response ameliorates ALS and FTD pathology in mouse and human models.
实验应用 : ICC/IF, IHC-Fr
种属反应 : Mouse
Hsu WH et al. Int J Biol Macromol 2020; 165 (Pt A) : 1604-1613 Effects of WSG, a polysaccharide from Ganoderma lucidum, on suppressing cell growth and mobility of lung cancer.
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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