产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000-1:10000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
vimentin
细胞定位
Cytoplasm,Cytoplasm, cytoskeleton
功能与背景
This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
数据库
研究领域
產品图片
![Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980](/templates/default/js/lightbox/images/loading.gif "Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980")
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GTX636980 WB Image
Wild-type (WT) and Vimentin knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636980 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX636980 IHC-P Image
Vimentin antibody [HL1506] detects Vimentin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636980 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636980 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX636980 IHC-P Image
Vimentin antibody [HL1506] detects Vimentin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100.Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636980 IHC-P Image
Vimentin antibody [HL1506] detects Vimentin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636980 IHC-P Image
Vimentin antibody [HL1506] detects Vimentin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded cat lymph node.Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636980 IHC-P Image
Vimentin antibody [HL1506] detects Vimentin protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissue.Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636980 IHC-P Image
Vimentin antibody [HL1506] detects Vimentin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissue.Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636980 ICC/IF Image
Vimentin antibody [HL1506] detects Vimentin protein at cytoskeleton and cell junction by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636980 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980](/upload/website/prouct_img/small/GTX636980/GTX636980_44725_20220819_WB_KO_watermark_22082402_854.webp "Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980")
![Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980](/upload/website/prouct_img/small/GTX636980/GTX636980_44725_20220812_WB_competitor_watermark_22081423_808.webp "Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980")
![Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980](/upload/website/prouct_img/small/GTX636980/GTX636980_T-44676_20220701_IHC-P_22070501_793.webp "Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980")
![Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980](/upload/website/prouct_img/small/GTX636980/GTX636980_44725_20220701_WB_22070501_628.webp "Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980")
![Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980](/upload/website/prouct_img/small/GTX636980/GTX636980_44725_20220701_WB_M_R_22070501_818.webp "Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980")
![Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980](/upload/website/prouct_img/small/GTX636980/GTX636980_44725_20220701_IHC-P_M_22071823_395.webp "Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980")
![Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980](/upload/website/prouct_img/small/GTX636980/GTX636980_44725_20220701_IHC-P_22071823_281.webp "Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980")
![Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980](/upload/website/prouct_img/small/GTX636980/GTX636980_44725_20230331_IHC-P_Cat_23032819_622.webp "Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980")
![Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980](/upload/website/prouct_img/small/GTX636980/GTX636980_44725_20221228_IHC-P_multiple_R_22122901_646.webp "Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980")
![Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980](/upload/website/prouct_img/small/GTX636980/GTX636980_44725_20221228_IHC-P_multiple_M_22122901_907.webp "Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980")
![Anti-Vimentin antibody [HL1506] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636980](/upload/website/prouct_img/small/GTX636980/GTX636980_T-44676_20220916_ICC_IF_22102723_567.webp "Anti-Vimentin antibody [HL1506] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636980")
![Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980](/upload/website/prouct_img/small/GTX636980/GTX636980_44725_20220812_WB_B_22081423_799.webp "Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980")
![Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980](/upload/website/prouct_img/normal/GTX636980/GTX636980_44725_20220819_WB_KO_watermark_22082402_854.webp "Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980")
![Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980](/upload/website/prouct_img/normal/GTX636980/GTX636980_44725_20220812_WB_competitor_watermark_22081423_808.webp "Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980")
![Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980](/upload/website/prouct_img/normal/GTX636980/GTX636980_T-44676_20220701_IHC-P_22070501_793.webp "Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980")
![Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980](/upload/website/prouct_img/normal/GTX636980/GTX636980_44725_20220701_WB_22070501_628.webp "Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980")
![Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980](/upload/website/prouct_img/normal/GTX636980/GTX636980_44725_20220701_WB_M_R_22070501_818.webp "Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980")
![Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980](/upload/website/prouct_img/normal/GTX636980/GTX636980_44725_20220701_IHC-P_M_22071823_395.webp "Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980")
![Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980](/upload/website/prouct_img/normal/GTX636980/GTX636980_44725_20220701_IHC-P_22071823_281.webp "Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980")
![Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980](/upload/website/prouct_img/normal/GTX636980/GTX636980_44725_20230331_IHC-P_Cat_23032819_622.webp "Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980")
![Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980](/upload/website/prouct_img/normal/GTX636980/GTX636980_44725_20221228_IHC-P_multiple_R_22122901_646.webp "Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980")
![Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980](/upload/website/prouct_img/normal/GTX636980/GTX636980_44725_20221228_IHC-P_multiple_M_22122901_907.webp "Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980")
![Anti-Vimentin antibody [HL1506] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636980](/upload/website/prouct_img/normal/GTX636980/GTX636980_T-44676_20220916_ICC_IF_22102723_567.webp "Anti-Vimentin antibody [HL1506] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636980")
![Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980](/upload/website/prouct_img/normal/GTX636980/GTX636980_44725_20220812_WB_B_22081423_799.webp "Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980")
