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ZWINT抗体

Anti-ZWINT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107155
Anti-ZWINT antibody used in Western Blot (WB). GTX107155
Anti-ZWINT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107155

目录号 GTX107155

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF

种属反应

Human
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

31 kDa. ( Note )

阳性对照

293T , Molt-4 , Raji

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human ZWINT. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1952700

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

ZW10 interacting kinetochore protein , HZwint-1 , KNTC2AP , SIP30 , ZWINT1

细胞定位

Nucleus , Kinetochore

功能与背景

This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-ZWINT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107155

GTX107155 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed 293T, using ZWINT(GTX107155) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

Anti-ZWINT antibody used in Western Blot (WB). GTX107155

GTX107155 WB Image

Sample(30 ug whole cell lysate)
A:293T
B:MOLT4 (GTX27912)
C:Raji (GTX27908)
12% SDS PAGE
GTX107155 diluted at 1:1000

Anti-ZWINT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107155

GTX107155 ICC/IF Image

ZWINT antibody detects ZWINT protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZWINT protein stained by ZWINT antibody (GTX107155) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

文献引用

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SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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