应用说明
Recommended Starting Dilutions:
For WB: Use at a dilution of 1:1000-1:3000.
For ICC/IF: Use at a dilution of 1:500-1:2000.
For IP: Use at a dilution of 1:50-1:200.
Optimal dilutions should be determined experimentally by the end user.
产品说明
Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
形式
Liquid
存储溶液
10mM PBS pH7.2
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
Batch dependent (Please refer to the vial label for the specific concentration.)
免疫原
HHHHHH (6x His) synthetic peptide conjugated to KLH
纯化方式
Protein A purified
偶联
Unconjugated
RRID
AB_423119
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
别名
6 His , His tag , 6 His epitope tag , HHHHHH tag , HHHHHH epitope tag
功能与背景
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
研究领域
![Anti-6X His tag antibody [HIS.H8] used in Western Blot (WB). GTX18184](/upload/website/prouct_img/small/GTX18184/6x-His-tag-antibody-HIS.H8-GTX18184-WB-1_w_23060620_677.webp "Anti-6X His tag antibody [HIS.H8] used in Western Blot (WB). GTX18184")
![Anti-6X His tag antibody [HIS.H8] used in Western Blot (WB). GTX18184](/upload/website/prouct_img/small/GTX18184/6x-His-tag-antibody-HIS.H8-GTX18184-WB-2_w_23060620_314.webp "Anti-6X His tag antibody [HIS.H8] used in Western Blot (WB). GTX18184")
![Anti-6X His tag antibody [HIS.H8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX18184](/upload/website/prouct_img/small/GTX18184/6x-His-tag-antibody-HIS.H8-GTX18184-ICC_IF-1_w_23060620_464.webp "Anti-6X His tag antibody [HIS.H8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX18184")
![Anti-6X His tag antibody [HIS.H8] used in Western Blot (WB). GTX18184](/upload/website/prouct_img/normal/GTX18184/6x-His-tag-antibody-HIS.H8-GTX18184-WB-1_w_23060620_677.webp "Anti-6X His tag antibody [HIS.H8] used in Western Blot (WB). GTX18184")
![Anti-6X His tag antibody [HIS.H8] used in Western Blot (WB). GTX18184](/upload/website/prouct_img/normal/GTX18184/6x-His-tag-antibody-HIS.H8-GTX18184-WB-2_w_23060620_314.webp "Anti-6X His tag antibody [HIS.H8] used in Western Blot (WB). GTX18184")
![Anti-6X His tag antibody [HIS.H8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX18184](/upload/website/prouct_img/normal/GTX18184/6x-His-tag-antibody-HIS.H8-GTX18184-ICC_IF-1_w_23060620_464.webp "Anti-6X His tag antibody [HIS.H8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX18184")
