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CGRP antibody

Anti-CGRP antibody used in IHC (Frozen sections) (IHC-Fr). GTX134005
Anti-CGRP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134005
Anti-CGRP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134005
Anti-CGRP antibody used in IHC (Paraffin sections) (IHC-P). GTX134005
Anti-CGRP antibody used in IHC (Paraffin sections) (IHC-P). GTX134005
Anti-CGRP antibody used in IHC (Paraffin sections) (IHC-P). GTX134005

Cat. No. GTX134005

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CGRP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Predict Reactivity

Human(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse CGRP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887168

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calcitonin/calcitonin-related polypeptide, alpha , CA , CGRP-1 , CGRP1 , Calc , Calc1 , Cgrp , Ct , Ctn

Cellular Localization

Secreted

Background

This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide (CGRP) and katacalcin. Alternative splicing of the mRNA results in multiple variants that encode either calcitonin or CGRP preproproteins. Post-translational processing of the calcitonin and CGRP propeptides results in either calcitonin and katacalcin, or CGRP, respectively. Calcitonin and katacalcin modulate calcium levels in the blood stream. CGRP can function as a vasodilator and play a role in the transmission of pain. The human homolog of CGRP was found to have antimicrobial activity. [provided by RefSeq, Mar 2015]

Database

Research Area

DATA IMAGES

Anti-CGRP antibody used in IHC (Frozen sections) (IHC-Fr). GTX134005

GTX134005 IHC-Fr Image

Calcitonin antibody detects Calcitonin protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: Calcitonin stained by Calcitonin antibody (GTX134005) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CGRP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134005

GTX134005 ICC/IF Image

CGRP antibody detects CGRP protein at cytoplasmic vesicles by immunofluorescent analysis.
Sample: mouse dorsal root ganglia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CGRP stained by CGRP antibody (GTX134005) diluted at 1:500.
Red: NF-H, a axon marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CGRP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134005

GTX134005 ICC/IF Image

Calcitonin antibody detects Calcitonin protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calcitonin stained by Calcitonin antibody (GTX134005) diluted at 1:500.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CGRP antibody used in IHC (Paraffin sections) (IHC-P). GTX134005

GTX134005 IHC-P Image

Calcitonin antibody detects Calcitonin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spinal cord .
Calcitonin stained by Calcitonin antibody (GTX134005) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CGRP antibody used in IHC (Paraffin sections) (IHC-P). GTX134005

GTX134005 IHC-P Image

Calcitonin antibody detects Calcitonin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Calcitonin stained by Calcitonin antibody (GTX134005) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CGRP antibody used in IHC (Paraffin sections) (IHC-P). GTX134005

GTX134005 IHC-P Image

Calcitonin antibody detects Calcitonin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Calcitonin stained by Calcitonin antibody (GTX134005) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Qingsong Ran et al. Front Endocrinol (Lausanne) 2020; 11 : 609697 Generation of Thyroid Tissues From Embryonic Stem Cells via Blastocyst Complementation In Vivo.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

CGRP antibody Cat. No. GTX134005

Rating
( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-CGRP antibody used in IHC (Paraffin sections) (IHC-P). GTX134005
Date : Anonymous submitted on 16-Nov-2022
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Mouse trigeminal ganglion
Blocking : 1% BSA in PBS, RT°C, 0.5Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.2% Triton-X100 in PBS / EDTA pH 8.0
Primary Antibody : 1:100, 2°C, RTHr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169