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ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187)抗体

Orthogonal
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX24819
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX24819
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX24819
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX24819
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX24819
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX24819

目录号 GTX24819

文献引用 30

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC, IHC (Free Floating)

种属反应

 Human, Mouse, Rat, Drosophila, Bovine, Chicken, Caenorhabditis elegans, Xenopus
产品包装
50 μl (¥5600)
查看所有 ERK1 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000
ICC/IF Assay dependent
IHC-P 1:10-1:100
IHC Assay dependent
IHC (Free Floating) Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品说明

The antibody recognizes the same sequence in both proteins (ERK1/2) as long as the T and the Y are phosphorylated. Does not recognize either monophospho- forms of ERK1/2 or the non-phosphorylated proteins.

产品属性

形式

Liquid

存储溶液

PBS, 0.1% BSA, 50% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

Batch dependent (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human ERK1 and 2 that contains threonine 202/185 and tyrosine 204/187. This region is conserved among many species including rat, mouse, cow, frog, snail, nematode, and fruit fly.

纯化方式

Purified by antigen-affinity chromatography

偶联

Unconjugated

RRID

AB_380440

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

mitogen-activated protein kinase 1 , mitogen-activated protein kinase 3 , ERK , ERK-2 , ERK2 , ERT1 , MAPK2 , P42MAPK , PRKM1 , PRKM2 , p38 , p40 , p41 , p41mapk , p42-MAPK , ERK-1 , ERK1 , ERT2 , HS44KDAP , HUMKER1A , P44ERK1 , P44MAPK , PRKM3 , p44-ERK1 , p44-MAPK

细胞定位

Cytoplasm,Nucleus

功能与背景

kinase involved in intracellular signaling; component of Mapk signaling pathway [RGD, Feb 2006]

数据库

研究领域

產品图片

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX24819

GTX24819 WB Image

Orthogonal

WB analysis of whole cell extracts (30 μg lysate) of MDA-MB-231 with treatment of EGF(100ng/ml for 15mins) (Lane 1), MDA-MB-231 (Lane 2), U-87 MG (Lane 3), SH-SY5Y (Lane 4) and HeLa (Lane 5) using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody.
Dilution : 1:1000

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX24819

GTX24819 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:50

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX24819

GTX24819 WB Image

Orthogonal

WB (peptide competition) analysis of PC12 cells stimulated with 0.5 M sorbitol for 5 minutes (Lane 2-6) using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphothreonine-containing peptide (Lane 4), a generic phosphotyrosine-containing peptide (Lane 5), or the phosphopeptide immunogen (Lane 6). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX24819

GTX24819 IHC-P Image

IHC-P analysis of mouse stomach tissue using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX24819

GTX24819 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX24819

GTX24819 WB Image

Orthogonal

WB analysis of NIH3T3 untreated (Lane 1) or treated with PDGF (Lane 2) using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody.
Loading: 5 ug

文献引用

Application Reference

Hung YH et al. Am J Cancer Res 2023; 13 (8) : 3417-3432 Pancreatic cancer cell-derived semaphorin 3A promotes neuron recruitment to accelerate tumor growth and dissemination.
Zhang X et al. Chin Herb Med 2022; 14 (4) : 554-562 Taxifolin attenuates inflammation via suppressing MAPK signal pathway in vitro and in silico analysis.
实验应用 : WB
种属反应 : Mouse
Iwashita J et al. Front Pharmacol 2022; 13 : 851374 Type IV collagen reduces MUC5AC secretion in the lungs of ovalbumin-sensitized mice.
Yan Sun et al. Immun Inflamm Dis 2022; 10 (7) : e641 TRPM7 promotes lipopolysaccharide-induced inflammatory dysfunction in renal tubular epithelial cells.
实验应用 : WB
种属反应 : Human
Jun Iwashita et al. Biochem Biophys Rep 2021; 28 : 101124 Integrin β1 subunit regulates cellular and secreted MUC5AC and MUC5B production in NCI-H292 human lung epithelial cells.
实验应用 : WB
种属反应 : Human
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Application Reference
Hung YH et al. Am J Cancer Res 2023; 13 (8):3417-3432 Pancreatic cancer cell-derived semaphorin 3A promotes neuron recruitment to accelerate tumor growth and dissemination.
Zhang X et al. Chin Herb Med 2022; 14 (4):554-562 Taxifolin attenuates inflammation via suppressing MAPK signal pathway in vitro and in silico analysis.
实验应用 : WB
种属反应 : Mouse
Iwashita J et al. Front Pharmacol 2022; 13:851374 Type IV collagen reduces MUC5AC secretion in the lungs of ovalbumin-sensitized mice.
Yan Sun et al. Immun Inflamm Dis 2022; 10 (7):e641 TRPM7 promotes lipopolysaccharide-induced inflammatory dysfunction in renal tubular epithelial cells.
实验应用 : WB
种属反应 : Human
Jun Iwashita et al. Biochem Biophys Rep 2021; 28:101124 Integrin β1 subunit regulates cellular and secreted MUC5AC and MUC5B production in NCI-H292 human lung epithelial cells.
实验应用 : WB
种属反应 : Human
Shih TL et al. J Cell Mol Med 2021; 25 (19):9434-9446 A novel naphthalimide derivative reduces platelet activation and thrombus formation via suppressing GPVI.
实验应用 : WB
种属反应 : Mouse
Li Y et al. Transl Lung Cancer Res 2021; 10 (4):1841-1856 Let-7b-3p inhibits tumor growth and metastasis by targeting the BRF2-mediated MAPK/ERK pathway in human lung adenocarcinoma.
实验应用 : WB
种属反应 : Human
Huang C et al. BMC Complement Med Ther 2020; 20 (1):68 Curcumin, demethoxycurcumin, and bisdemethoxycurcumin induced caspase-dependent and -independent apoptosis via Smad or Akt signaling pathways in HOS cells.
Navarrete-Bernal MGC et al. Front Oncol 2020; 10:1206 Biological Landscape of Triple Negative Breast Cancers Expressing CTLA-4.
实验应用 : WB
种属反应 : Human
Wang CH et al. Sci Rep 2020; 10 (1):2187 The novel application of cordycepin in maintaining stem cell pluripotency and increasing iPS cell generation efficiency.
实验应用 : WB
种属反应 : Mouse
Yang SS et al. Bone 2019; 130:115073 Tauroursodeoxycholic acid induces angiogenic activity in endothelial cells and accelerates bone regeneration.
实验应用 : WB
种属反应 : Human
Chen YR et al. Food Chem Toxicol 2019; 132:110693 Thyroid hormone-induced expression of inflammatory cytokines interfere with resveratrol-induced anti-proliferation of oral cancer cells.
实验应用 : WB
种属反应 : Human
Huang HY et al. Fish Shellfish Immunol 2019; 90:338-348 Dietary N-Carbamylglutamate (NCG) alleviates liver metabolic disease and hepatocyte apoptosis by suppressing ERK1/2-mTOR-S6K1 signal pathway via promoting endogenous arginine synthesis in Japanese seabass (Lateolabrax japonicus).
实验应用 : WB
Jau?Tien Lin et al. Journal of Food Biochemistry 2019; (Epub) Litchi (Litchi chinensis Sonn.) flower proanthocyanidin fraction exhibited protective efficacy to suppress nickel?induced expression for vascular endothelial growth factor in HepG2 cells.
实验应用 : WB
种属反应 : Human
Hsieh WY et al. PLoS One 2019; 14 (1):e0210656 Renal chymase-dependent pathway for angiotensin II formation mediated acute kidney injury in a mouse model of aristolochic acid I-induced acute nephropathy.
实验应用 : WB
种属反应 : Mouse
Liu W et al. J Anesth 2018; (Epub) effects of hypothermia during * anesthesia on learning and memory ability and hippocampal apoptosis in neonatal rats.
实验应用 : WB
种属反应 : Rat
Huang KH et al. J Endod 2018; 44 (11):1677-1685 Enhanced Capability of Bone Morphogenetic Protein 2-loaded Mesoporous Calcium Silicate Scaffolds to Induce Odontogenic Differentiation of Human Dental Pulp Cells.
实验应用 : WB
种属反应 : Human
Chen CF et al. Oncology Letters 2018;:6323-6330 synergistic inhibitory effects of * and curcumin on human *-resistant oral cancer car cells through intrinsic apoptotic process.
实验应用 : WB
种属反应 : Human
Li JM et al. Ther Adv Med Oncol 2018; 10:1.76E+15 Upregulation of LGALS1 is associated with oral cancer metastasis.
实验应用 : WB
种属反应 : Human
Lin JT et al. Journal of Functional Foods 2018; 47:343-349 Protective effect and mechanism of Muntingia calabura Linn. fruit ethanolic extract against vascular endothelial growth factor production in nickel-stimulated hepatocellular carcinoma cells.
实验应用 : WB
种属反应 : Human
Lin C.I. et al. Int. J. Biol. Sci. 2018; 14 (3):253-265 Instillation of particulate matter 2.5 induced acute lung injury and attenuated the injury recovery in ACE2 knockout mice.
实验应用 : WB
种属反应 : Mouse
Yeh CJ et al. Sci Rep 2017; 7 (1):15599 The effects of artocarpin on wound healing: in vitro and in vivo studies.
实验应用 : WB
种属反应 : Human
Cai H et al. J Nat Med 2017; (Epub) 18β-glycyrrhetinic acid inhibits migration and invasion of human gastric cancer cells via the ROS/PKC-α/ERK pathway.
实验应用 : WB
种属反应 : Human
Lin WC et al. RSC Adv. 2017; 7:21234-21251 Anti-inflammatory activity of Sanghuangporus sanghuang by suppressing the TLR4-mediated PI3K/AKT/mTOR/IKKβ signaling pathway.
实验应用 : WB
种属反应 : Mouse
Chen CC et al. PLoS One 2016; 11 (7):e0158662 The Anti-Inflammatory Effects and Mechanisms of Eupafolin in Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Macrophages.
实验应用 : WB
种属反应 : Mouse
Chen WL et al. PLoS One 2015; 10 (9):e0137979 Mycobacterium tuberculosis Upregulates TNF-α Expression via TLR2/ERK Signaling and Induces MMP-1 and MMP-9 Production in Human Pleural Mesothelial Cells.
实验应用 : WB
种属反应 : Human
Vazquez-Mellado MJ et al. Life Sci 2015; 137 (Epub):142-9 erythropoietin protects neuroblastoma cells against * and * by activating erk and akt pathways but has no effect in kidney cells.
实验应用 : WB
种属反应 : Human
Clone Reference
Robinson FL et al. J Biol Chem 2002; 277 (17):14844-52 Identification of novel point mutations in ERK2 that selectively disrupt binding to MEK1.
Tong T et al. Exp Cell Res 2001; 269 (1):64-72 Involvement of the MAP kinase pathways in induction of GADD45 following UV radiation.
Janulis M et al. Mol Cell Biol 2001; 21 (6):2235-47 A novel mitogen-activated protein kinase is responsive to Raf and mediates growth factor specificity.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 5600
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