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Iba1抗体 [HL22]

Recombinant antibody Comparison Orthogonal
Anti-Iba1 antibody [HL22] used in IHC (Frozen sections) (IHC-Fr). GTX635363
Anti-Iba1 antibody [HL22] used in Western Blot (WB). GTX635363
Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363
Anti-Iba1 antibody [HL22] used in IHC (Frozen sections) (IHC-Fr). GTX635363
Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363
Anti-Iba1 antibody [HL22] used in IHC (Frozen sections) (IHC-Fr). GTX635363
Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363
Anti-Iba1 antibody [HL22] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635363
Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363
Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363
Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363
Anti-Iba1 antibody [HL22] used in Western Blot (WB). GTX635363
Anti-Iba1 antibody [HL22] used in Western Blot (WB). GTX635363

目录号 GTX635363

文献引用 10
4

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL22

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Iba1 的产品
产品说明

摘要

Iba1 antibody detects Iba1, a protein widely used by neuroscientists as a microglial marker and to gauge microglial activation. It is also expressed in macrophages. Discrimination between these two cell populations can be achieved via double staining with TMEM119 antibody (GTX134087), another useful microglia-specific marker, by identifying brain-residing microglia as Iba1+/TMEM119+ while CNS-infiltrating macrophages are Iba1+/TMEM119-.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

17 kDa. ( Note )

阳性对照

THP-1 , HL-60

预测种属反应

Bovine, Pig, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Iba1. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2888516

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

allograft inflammatory factor 1 , AIF-1 , IBA1 , IRT-1 , IRT1

细胞定位

Cytoplasm, cytoskeleton

功能与背景

This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]

数据库

研究领域

產品图片

Anti-Iba1 antibody [HL22] used in IHC (Frozen sections) (IHC-Fr). GTX635363

GTX635363 IHC-Fr Image

Comparison

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse spinal cord.
Green: Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Iba1 antibody [HL22] used in Western Blot (WB). GTX635363

GTX635363 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Iba1 antibody [HL22] (GTX635363) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363

GTX635363 IHC-P Image

Comparison

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody [HL22] used in IHC (Frozen sections) (IHC-Fr). GTX635363

GTX635363 IHC-Fr Image

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse brain.
Green: Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:100.
Red: Iba1 stained by Iba1 antibody [HL22-RT] (GTX635400) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363

GTX635363 IHC-P Image

Comparison

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody [HL22] used in IHC (Frozen sections) (IHC-Fr). GTX635363

GTX635363 IHC-Fr Image

Iba1 antibody [HL22] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Frozen-sectioned mouse spinal cord.
Green: Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363

GTX635363 IHC-P Image

Iba1 antibody [HL22] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody [HL22] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635363

GTX635363 ICC/IF Image

Iba1 antibody [HL22] detects Iba1 protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363

GTX635363 IHC-P Image

Iba1 antibody [HL22] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363

GTX635363 IHC-P Image

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 stained by Iba1 antibody [HL22] (GTX635363) conjugated with DyLight488 diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363

GTX635363 IHC-P Image

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Green: Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:150.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Iba1 antibody [HL22] used in Western Blot (WB). GTX635363

GTX635363 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [HL22] (GTX635363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Iba1 antibody [HL22] used in Western Blot (WB). GTX635363

GTX635363 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [HL22] (GTX635363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Brown TC et al. bioRxiv 2023; Microglia are dispensable for experience-dependent refinement of visual circuitry.
Wang HK et al. J Inflamm Res 2023; 16 : 4103-4116 HDAC1 is Involved in Neuroinflammation and Blood-Brain Barrier Damage in Stroke Pathogenesis.
Wang HK et al. J Inflamm Res 2023; 2023 (16) : 4103—4116 HDAC1 is Involved in Neuroinflammation and Blood-Brain Barrier Damage in Stroke Pathogenesis
实验应用 : IHC-Fr
种属反应 : Rat
Iacono D et al. Sci Rep 2023; 13 (1) : 21142 Low-dose brain radiation: lowering hyperphosphorylated-tau without increasing DNA damage or oncogenic activation.
Chia-En Wong et al. J Neuroinflammation 2022; 19 (1) : 153 Sciatic nerve stimulation alleviates acute neuropathic pain via modulation of neuroinflammation and descending pain inhibition in a rodent model.
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产品评论

Iba1 antibody [HL22] 目录号 GTX635363

Rating
( Average 5 based on 4 users reviews)
实验应用
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 3 users reviews)
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363
Date : Anonymous submitted on 13-Apr-2022
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : human Brain tissue section
Permeabilization /Antigen retrieval : Tris-EDTA pH9.0
Primary Antibody : 1:1000, 25°C, 1Hr
Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363
Date : Anonymous submitted on 18-Oct-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : mouse brain
Primary Antibody : 1:150, 4°C, 16Hr
Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363
Date : Wei-Hao Liao submitted on 21-Sep-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Mouse hippocampus
Fixation : 4% PFA in PBS, 4ºC, overnight
Permeabilization /Antigen retrieval : citric acid (pH6), 25min, 95°C
Primary Antibody : 1:100, 4°C, 16Hr
Anti-Iba1 antibody [HL22] used in IHC (Frozen sections) (IHC-Fr). GTX635363
Date : Anonymous submitted on 16-Jun-2020
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : Brain
Blocking : Donkey serum, 25°C, 1Hr
Primary Antibody : 1:300, 4°C, 16Hr
我要评论产品
Application Reference
Brown TC et al. bioRxiv 2023; Microglia are dispensable for experience-dependent refinement of visual circuitry.
Wang HK et al. J Inflamm Res 2023; 16:4103-4116 HDAC1 is Involved in Neuroinflammation and Blood-Brain Barrier Damage in Stroke Pathogenesis.
Wang HK et al. J Inflamm Res 2023; 2023 (16):4103—4116 HDAC1 is Involved in Neuroinflammation and Blood-Brain Barrier Damage in Stroke Pathogenesis
实验应用 : IHC-Fr
种属反应 : Rat
Iacono D et al. Sci Rep 2023; 13 (1):21142 Low-dose brain radiation: lowering hyperphosphorylated-tau without increasing DNA damage or oncogenic activation.
Chia-En Wong et al. J Neuroinflammation 2022; 19 (1):153 Sciatic nerve stimulation alleviates acute neuropathic pain via modulation of neuroinflammation and descending pain inhibition in a rodent model.
Fan Z et al. Ecotoxicol Environ Saf 2022; 234:113372 JAK2/STAT3 pathway regulates microglia polarization involved in hippocampal inflammatory damage due to acute paraquat exposure.
实验应用 : WB
种属反应 : Rat
Zhang Y et al. Cell Death Dis 2021; 12 (12):1159 TRPV1 channel mediates NLRP3 inflammasome-dependent neuroinflammation in microglia.
实验应用 : IHC-P
种属反应 : Mouse
Yan H et al. Neuropharmacology 2021; 194:108627 Parecoxib alleviates the motor behavioral decline of aged rats by ameliorating mitochondrial dysfunction in the substantia nigra via COX-2/PGE2 pathway inhibition.
实验应用 : IHC-P
种属反应 : Rat
Xiong LL et al. Am J Chin Med 2021; 49 (3):677-703 Effect of Sutellarin on Neurogenesis in Neonatal Hypoxia-Ischemia Rat Model: Potential Mechanisms of Action.
Teng SW et al. Cell Biol Int 2021; 45 (5):1072-1081 Potential surrogate quantitative immunomodulatory potency assay for monitoring human umbilical cord-derived mesenchymal stem cells production.
实验应用 : IHC-P
SDS
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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