产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
Assay dependent |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody was raised against human RAI3 Intracellular domain.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the Intracellular domain of human RAI3. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
G protein-coupled receptor class C group 5 member A , GPCR5A , PEIG-1 , RAI3 , RAIG1 , TIG1
细胞定位
Cell membrane
功能与背景
This gene encodes a member of the type 3 G protein-coupling receptor family, characterized by the signature 7-transmembrane domain motif. The encoded protein may be involved in interaction between retinoid acid and G protein signalling pathways. Retinoic acid plays a critical role in development, cellular growth, and differentiation. This gene may play a role in embryonic development and epithelial cell differentiation. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX637589 WB Image
Unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (GTX637589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637589 WB Image
Non-transfected (–) and transfected (+) unbiled MCF-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with RAI3 antibody [HL1864] (GTX637589) diluted at 1:5000 and the highly cited competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX637589 WB Image
Wild-type (WT) and RAI3 knockout (KO) MCF-7 cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with RAI3 antibody [HL1864] (GTX637589) diluted at 1:5000 and competitor's antibody (Competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX637589 IHC-P Image
RAI3 antibody [HL1864] detects RAI3 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.RAI3 stained by RAI3 antibody [HL1864] (GTX637589) diluted at 1:100 and competitor's antibody (10309-1-AP) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min *Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
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GTX637589 IHC-P Image
RAI3 antibody [HL1864] detects RAI3 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.RAI3 stained by RAI3 antibody [HL1864] (GTX637589) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637589 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (GTX637589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX637589 WB Image
Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (GTX637589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637589 ICC/IF Image
RAI3 antibody [HL1864] detects RAI3 protein at cell membrane by immunofluorescent analysis.Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: RAI3 stained by RAI3 antibody [HL1864] (GTX637589) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637589 IHC-P Image
RAI3 antibody [HL1864] detects RAI3 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded human kidney.RAI3 stained by RAI3 antibody [HL1864] (GTX637589) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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