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S Tag抗体

Anti-S Tag antibody used in Immunoprecipitation (IP). GTX128060
Anti-S Tag antibody used in Western Blot (WB). GTX128060
Anti-S Tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128060

目录号 GTX128060

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IP

种属反应

Species independent
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF Assay dependent
IP 2.5 ug
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.5 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原

The immunogen used to generate this antibody corresponds to S Tag (KETAAAKFERQHMDS).

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2885696

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

產品图片

Anti-S Tag antibody used in Immunoprecipitation (IP). GTX128060

GTX128060 IP Image

S-tag antibody immunoprecipitates S-tag protein in IP experiments. IP Sample: 650 μg whole cell lysate/extract of S-tag protein expressing HeLa cells. A. 20 μg whole cell lysate/extract of transfected HeLa cells B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of S-tag protein by 2.5 μg of S-tag antibody (GTX128060) 12% SDS-PAGE The immunoprecipitated S-tag protein was detected by S-tag antibody (GTX128060) diluted at 1:10000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-S Tag antibody used in Western Blot (WB). GTX128060

GTX128060 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with S Tag antibody (GTX128060) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-S Tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128060

GTX128060 ICC/IF Image

S Tag antibody detects S protein by immunofluorescent analysis.
Sample: S-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: S stained by S Tag antibody (GTX128060) diluted at 1:2000.
Blue: Hoechst 33342 staining.

文献引用

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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