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Streptavidin (HRP)

Streptavidin (HRP). GTX27403
Streptavidin (HRP). GTX27403
Streptavidin (HRP). GTX27403

目录号 GTX27403

实验应用

WB, IHC-P, IHC-Fr, Dot, ELISA, IM
产品包装
500 μg (¥2400)
产品说明

摘要

Streptavidin is a tetrameric protein capable of binding 4 biotin groups to each molecule of streptavidin. While streptavidin has identical binding properties as avidin, it lacks the glycoprotein portion of the molecule and therefore shows less non-specific binding. Streptavidin is a slightly smaller molecule with a molecular weight of approximately 53.6 kDa. The sequence of avidin only shows 30% homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
Dot Assay dependent
ELISA Assay dependent
IM Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品属性

形式

Liquid

存储溶液

0.02M Potassium Phosphate (pH7.2), 0.15M Sodium Chloride

保存剂

No preservative

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

表达系统

Streptomyces avidinii

纯化方式


This product was prepared from chromatographically purified streptavidin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin. No reaction was observed against anti-Avidin.

偶联

Horseradish peroxidase(HRP)

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

產品图片

Streptavidin (HRP). GTX27403

GTX27403 ELISA Image

Detection of Rabbit anti Mouse TNFα:biotin (GTX74045) in a sandwich ELISA with recombinant murine TNFα as the antigen and Rat anti Mouse TNFα as the coating antibody using Streptavidin:HRP (GTX27403)

Streptavidin (HRP). GTX27403

GTX27403 WB Image

Western Blot of Peroxidase Conjugated Streptavidin. Lane 1: Human IL-7. Lane 2: none. Load: 50 ng per lane. Primary antibody: Human IL-7 Biotin Conjugated antibody (GTX27403) at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase Conjugated Streptavidin at 1:40,000 for 30 min at RT. Block: 5% BLOTTO 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for Human IL-7. Other band(s): none

Streptavidin (HRP). GTX27403

GTX27403 WB Image

Detection of Mouse anti histidine tag:biotin (GTX74967) in a Western blot analysis of a HIS tagged protein using Streptavidin:HRP (GTX27403)

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产品包装 报价 (¥)
¥ 2400
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