产品说明
摘要
14-3-3 antibody detects 14-3-3 proteins, which in mammals exist in seven isoforms (β, γ, ε, η, ζ, σ, and τ/θ) and have predicted molecular weights of ~28 kDa. 14-3-3 proteins are homo- or heterodimeric adaptors involved in the regulation of various signaling pathways such as mTOR, AMPK, and MAPK.
产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
293T , A431 , HeLa , HepG2
预测种属反应
Rat, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.19 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the N-terminus region of human 14-3-3 beta. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2887086
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
GW128 antibody , HS1 antibody , KCIP1 antibody , YWHAA antibody , YWHAB antibody , tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta antibody
细胞定位
Cytoplasm , Melanosome
功能与背景
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq]
研究领域
產品图片
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GTX133736 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 antibody (GTX133736) diluted at 1:3000 and competitor's antibody (sc-629) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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文献引用
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产品评论
14-3-3 antibody
目录号 GTX133736
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Rating
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( Average 5 based on 1 users reviews)
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实验应用
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Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 12-Jan-2018
Application Tested :
WB
Sample Species :
Ms
Sample :
C2C12 cells
Amount used :
40 μg
Blocking :
1% BSA in TBS, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
